Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model

Eleanor Townsend, Leighann Sherry, Ranjith Rajendran, Donald Hansom, John Butcher, William MacKay, Craig Williams, Gordon Ramage

Research output: Contribution to journalArticle

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Abstract

Chronic diabetic foot ulcers are frequently colonised and infected by polymicrobial biofilms that ultimately prevent healing. This study aimed to create a novel in vitro inter-kingdom wound biofilm model on complex hydrogel-based cellulose substrata to test commonly used topical wound treatments. Inter-kingdom triadic biofilms composed of Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus were shown to be quantitatively greater in this model compared to a simple substratum when assessed by conventional culture, metabolic dye and live dead qPCR. These biofilms were both structurally complex and compositionally dynamic in response to topical therapy, so when treated with either chlorhexidine or povidone iodine, principal component analysis revealed that the 3-D cellulose model was minimally impacted compared to the simple substratum model. This study highlights the importance of biofilm substratum and inclusion of relevant polymicrobial and inter-kingdom components, as these impact penetration and efficacy of topical antiseptics.
Original languageEnglish
Pages (from-to)1259-1270
Number of pages11
JournalBiofouling - The Journal of Bioadhesion and Biiofilm
Volume32
Issue number10
Early online date14 Nov 2016
DOIs
Publication statusE-pub ahead of print - 14 Nov 2016

Fingerprint

Biofilms
biofilm
Wounds and Injuries
Cellulose
cellulose
wound treatment
chlorhexidine
Povidone-Iodine
antiseptics
Diabetic Foot
Local Anti-Infective Agents
Chlorhexidine
Hydrogel
hydrocolloids
iodine
Principal Component Analysis
Candida albicans
Pseudomonas aeruginosa
dyes
Staphylococcus aureus

Keywords

  • Chronic wound
  • in vitro model
  • polymicrobial
  • inter kingdom
  • biofilm

Cite this

Townsend, Eleanor ; Sherry, Leighann ; Rajendran, Ranjith ; Hansom, Donald ; Butcher, John ; MacKay, William ; Williams, Craig ; Ramage, Gordon. / Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. In: Biofouling - The Journal of Bioadhesion and Biiofilm. 2016 ; Vol. 32, No. 10. pp. 1259-1270.
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Development and characterisation of a novel three-dimensional inter-kingdom wound biofilm model. / Townsend, Eleanor; Sherry, Leighann; Rajendran, Ranjith; Hansom, Donald; Butcher, John; MacKay, William; Williams, Craig; Ramage, Gordon.

In: Biofouling - The Journal of Bioadhesion and Biiofilm, Vol. 32, No. 10, 14.11.2016, p. 1259-1270.

Research output: Contribution to journalArticle

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AU - Sherry, Leighann

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AU - MacKay, William

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