Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites

Fedia Ben Mefteh, Amal Daoud, Ali Chenari Bouket, Bathini Thissera, Yamina Kadri, Hafsa Cherif-Silini, Manal Eshelli, Faizah N Alenezi, Armelle Vallat, Tomasz Oszako, Adel Kadri, José María Ros-García, Mostafa E Rateb, Neji Gharsallah, Lassaad Belbahri

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography⁻High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography⁻Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.

Original languageEnglish
Article number1986
JournalInternational Journal of Molecular Sciences
Volume19
Issue number7
DOIs
Publication statusPublished - 7 Jul 2018

Fingerprint

Endophytes
metabolites
Metabolites
activity (biology)
industrial plants
Industrial plants
Assays
Trichoderma
Fusarium
bacteria
acetates
Bacteria
Inhibitory Concentration 50
fungi
Mass Spectrometry
Fungi
Anti-Inflammatory Agents
Bioactivity
cells
Geotrichum

Cite this

Ben Mefteh, F., Daoud, A., Chenari Bouket, A., Thissera, B., Kadri, Y., Cherif-Silini, H., ... Belbahri, L. (2018). Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites. International Journal of Molecular Sciences, 19(7), [1986]. https://doi.org/10.3390/ijms19071986
Ben Mefteh, Fedia ; Daoud, Amal ; Chenari Bouket, Ali ; Thissera, Bathini ; Kadri, Yamina ; Cherif-Silini, Hafsa ; Eshelli, Manal ; Alenezi, Faizah N ; Vallat, Armelle ; Oszako, Tomasz ; Kadri, Adel ; Ros-García, José María ; Rateb, Mostafa E ; Gharsallah, Neji ; Belbahri, Lassaad. / Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 7.
@article{8fd29e6312cd4f9eb74459887415947b,
title = "Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites",
abstract = "Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography⁻High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography⁻Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.",
author = "{Ben Mefteh}, Fedia and Amal Daoud and {Chenari Bouket}, Ali and Bathini Thissera and Yamina Kadri and Hafsa Cherif-Silini and Manal Eshelli and Alenezi, {Faizah N} and Armelle Vallat and Tomasz Oszako and Adel Kadri and Ros-Garc{\'i}a, {Jos{\'e} Mar{\'i}a} and Rateb, {Mostafa E} and Neji Gharsallah and Lassaad Belbahri",
year = "2018",
month = "7",
day = "7",
doi = "10.3390/ijms19071986",
language = "English",
volume = "19",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "7",

}

Ben Mefteh, F, Daoud, A, Chenari Bouket, A, Thissera, B, Kadri, Y, Cherif-Silini, H, Eshelli, M, Alenezi, FN, Vallat, A, Oszako, T, Kadri, A, Ros-García, JM, Rateb, ME, Gharsallah, N & Belbahri, L 2018, 'Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites', International Journal of Molecular Sciences, vol. 19, no. 7, 1986. https://doi.org/10.3390/ijms19071986

Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites. / Ben Mefteh, Fedia; Daoud, Amal; Chenari Bouket, Ali; Thissera, Bathini; Kadri, Yamina; Cherif-Silini, Hafsa; Eshelli, Manal; Alenezi, Faizah N; Vallat, Armelle; Oszako, Tomasz; Kadri, Adel; Ros-García, José María; Rateb, Mostafa E; Gharsallah, Neji; Belbahri, Lassaad.

In: International Journal of Molecular Sciences, Vol. 19, No. 7, 1986, 07.07.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Date palm trees root-derived endophytes as fungal cell factories for diverse bioactive metabolites

AU - Ben Mefteh, Fedia

AU - Daoud, Amal

AU - Chenari Bouket, Ali

AU - Thissera, Bathini

AU - Kadri, Yamina

AU - Cherif-Silini, Hafsa

AU - Eshelli, Manal

AU - Alenezi, Faizah N

AU - Vallat, Armelle

AU - Oszako, Tomasz

AU - Kadri, Adel

AU - Ros-García, José María

AU - Rateb, Mostafa E

AU - Gharsallah, Neji

AU - Belbahri, Lassaad

PY - 2018/7/7

Y1 - 2018/7/7

N2 - Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography⁻High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography⁻Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.

AB - Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography⁻High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography⁻Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.

U2 - 10.3390/ijms19071986

DO - 10.3390/ijms19071986

M3 - Article

C2 - 29986518

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 7

M1 - 1986

ER -