Cytotoxic potential of Nephthea sp.-derived actinomycetes supported by metabolomics analysis

Omnia Hesham Abdelhafez; John Refaat Fahim; Mostafa E. Rateb; Che J. Ngwa; Gabriele Pradel; Usama Ramadan Abdelmohsen

doi:10.1080/14786419.2022.2039650

Cytotoxic potential of Nephthea sp.-derived actinomycetes supported by metabolomics analysis

Omnia Hesham Abdelhafez, John Refaat Fahim, Mostafa E. Rateb, Che J. Ngwa, Gabriele Pradel, Usama Ramadan Abdelmohsen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Soft corals and associated microorganisms are known to produce leads for anticancer drugs. Keeping this in mind, Nephthea sp.; a Red Sea soft coral was investigated for the first time using the OSMAC approach. Two isolates, Streptomyces sp. UR63 and Micrococcus sp. UR67 were identified. Their extracts revealed the presence of alkaloids, macrolides, quinones, fatty acids and terpenoids. Further comparison through a set of multivariate data analyses revealed their unique chemical profiles. The extracts displayed inhibitory potencies against HepG-2, Caco-2 and MCF-7 tumor cell lines with IC₅₀ values ranging from 11.4 to 38.7 μg/mL when compared with the positive control, doxorubicin. The study not only highlights the cytotoxic potential of soft coral-associated actinomycetes but also shows the advantage of using the OSMAC approach in this regard.

Original language	English
Pages (from-to)	6464-6469
Number of pages	6
Journal	Natural Product Research
Volume	36
Issue number	24
Early online date	17 Feb 2022
DOIs	https://doi.org/10.1080/14786419.2022.2039650
Publication status	Published - 17 Dec 2022

Keywords

actinomycetes
cytotoxic activity
metabolic profiling
Micrococcus
Nephthea
OSMAC
Streptomyces

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10.1080/14786419.2022.2039650

2022 02 02 Abdelhafez et al Cytotoxic acceptedAccepted author manuscript, 1.49 MB

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title = "Cytotoxic potential of Nephthea sp.-derived actinomycetes supported by metabolomics analysis",

abstract = "Soft corals and associated microorganisms are known to produce leads for anticancer drugs. Keeping this in mind, Nephthea sp.; a Red Sea soft coral was investigated for the first time using the OSMAC approach. Two isolates, Streptomyces sp. UR63 and Micrococcus sp. UR67 were identified. Their extracts revealed the presence of alkaloids, macrolides, quinones, fatty acids and terpenoids. Further comparison through a set of multivariate data analyses revealed their unique chemical profiles. The extracts displayed inhibitory potencies against HepG-2, Caco-2 and MCF-7 tumor cell lines with IC50 values ranging from 11.4 to 38.7 μg/mL when compared with the positive control, doxorubicin. The study not only highlights the cytotoxic potential of soft coral-associated actinomycetes but also shows the advantage of using the OSMAC approach in this regard.",

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author = "Abdelhafez, {Omnia Hesham} and Fahim, {John Refaat} and Rateb, {Mostafa E.} and Ngwa, {Che J.} and Gabriele Pradel and Abdelmohsen, {Usama Ramadan}",

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T1 - Cytotoxic potential of Nephthea sp.-derived actinomycetes supported by metabolomics analysis

AU - Abdelhafez, Omnia Hesham

AU - Fahim, John Refaat

AU - Rateb, Mostafa E.

AU - Ngwa, Che J.

AU - Pradel, Gabriele

AU - Abdelmohsen, Usama Ramadan

PY - 2022/12/17

Y1 - 2022/12/17

N2 - Soft corals and associated microorganisms are known to produce leads for anticancer drugs. Keeping this in mind, Nephthea sp.; a Red Sea soft coral was investigated for the first time using the OSMAC approach. Two isolates, Streptomyces sp. UR63 and Micrococcus sp. UR67 were identified. Their extracts revealed the presence of alkaloids, macrolides, quinones, fatty acids and terpenoids. Further comparison through a set of multivariate data analyses revealed their unique chemical profiles. The extracts displayed inhibitory potencies against HepG-2, Caco-2 and MCF-7 tumor cell lines with IC50 values ranging from 11.4 to 38.7 μg/mL when compared with the positive control, doxorubicin. The study not only highlights the cytotoxic potential of soft coral-associated actinomycetes but also shows the advantage of using the OSMAC approach in this regard.

AB - Soft corals and associated microorganisms are known to produce leads for anticancer drugs. Keeping this in mind, Nephthea sp.; a Red Sea soft coral was investigated for the first time using the OSMAC approach. Two isolates, Streptomyces sp. UR63 and Micrococcus sp. UR67 were identified. Their extracts revealed the presence of alkaloids, macrolides, quinones, fatty acids and terpenoids. Further comparison through a set of multivariate data analyses revealed their unique chemical profiles. The extracts displayed inhibitory potencies against HepG-2, Caco-2 and MCF-7 tumor cell lines with IC50 values ranging from 11.4 to 38.7 μg/mL when compared with the positive control, doxorubicin. The study not only highlights the cytotoxic potential of soft coral-associated actinomycetes but also shows the advantage of using the OSMAC approach in this regard.

KW - actinomycetes

KW - cytotoxic activity

KW - metabolic profiling

KW - Micrococcus

KW - Nephthea

KW - OSMAC

KW - Streptomyces

U2 - 10.1080/14786419.2022.2039650

DO - 10.1080/14786419.2022.2039650

M3 - Article

SN - 1478-6419

VL - 36

SP - 6464

EP - 6469

JO - Natural Product Research

JF - Natural Product Research

IS - 24

ER -

Cytotoxic potential of Nephthea sp.-derived actinomycetes supported by metabolomics analysis

Abstract

Keywords

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