Cytokine-Induced MMP13 Expression in Human Chondrocytes is dependent on Activating Transcription Factor 3 (ATF3) regulation

Chun Ming Chan, Christopher D. Macdonald, Gary Litherland, David J. Wilkinson, Andrew Skelton, G. Nicholas Europe-Finner, Andrew D. Rowan

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Abstract

Irreversible breakdown of cartilage extracellular matrix (ECM) by the collagenase matrix metalloproteinase 13 (MMP13) represents a key event in osteoarthritis (OA) progression. Although inflammation is most commonly associated with inflammatory joint diseases, it also occurs in OA and is thus relevant to the prevalent tissue destruction. Here, inflammation generates a cFOS AP-1 early response which indirectly affects MMP13 gene expression. To ascertain a more a direct effect on prolonged MMP13 production we examined the potential molecular events occurring between the rapid, transient expression of cFOS and the subsequent MMP13 induction. Importantly, we show MMP13 mRNA expression is mirrored by nascent hnRNA transcription. Employing ChIP assays, cFOS recruitment to the MMP13 promoter occurs at an early stage prior to gene transcription and that recruitment of transcriptional initiation markers also correlated with MMP13 expression. Moreover, protein synthesis inhibition following early cFOS expression resulted in a significant decrease in MMP13 expression thus indicating a role for different regulatory factors modulating expression of the gene. Subsequent mRNA transcriptome analyses highlighted several genes induced soon after cFOS that could contribute to MMP13 expression. Specific small interfering RNA-mediated silencing highlighted that ATF3 was as highly selective for MMP13 as cFOS. Moreover, ATF3 expression was AP-1(cFOS/cJUN)-dependent and expression levels were maintained after the early transient cFOS response. Furthermore, ATF3 bound the proximal MMP13 AP-1 motif in stimulated chondrocytes at time-points that no longer supported binding of cFOS. Consequently, these findings support roles for both cFOS (indirect) and ATF3 (direct) in effecting MMP13 transcription in human chondrocytes.
Original languageEnglish
JournalJournal of Biological Chemistry
Early online date12 Dec 2016
DOIs
Publication statusE-pub ahead of print - 12 Dec 2016

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    Cytokine-induced cysteine- serine-rich nuclear protein-1 (CSRNP1) selectively contributes to MMP1 expression in human chondrocytes

    Macdonald, C. D., Falconer, A. M. D., Chan, C. M., Wilkinson, D. J., Skelton, A., Reynard, L., Litherland, G. J., Europe-Finner, G. N. & Rowan, A. D., 15 Nov 2018, In : PLoS ONE. 13, 11, 16 p., e0207240.

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    Protein kinase D3 modulates MMP1 and MMP13 expression in human chondrocytes

    Baker, J., Falconer, A., Wilkinson, D. J., Europe-Finner, G. N., Litherland, G. & Rowan, A. D., 13 Apr 2018, In : PLoS ONE. 13, 4, 19 p., e0195864.

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  • 9 Citations (Scopus)

    Tribbles and arthritis: what are the links?

    Rowan, A. D. & Litherland, G. J., Oct 2015, In : Biochemical Society Transactions. 43, p. 1051-1056

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