Cumulative impact of anti-sea lice treatment (azamethiphos) on health status of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) in aquaculture

Josip Barisic, Stuart Cannon, Brian Quinn

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Abstract

Despite its widespread use in aquaculture, the impact of chemical anti-sea lice treatment on salmonids following application in a commercial farm has not been previously reported. This work reports the cumulative effect of three consecutive anti-sea lice treatments using azamethiphos on the health status of aquaculture reared rainbow trout through the investigation of clinical chemistry, histopathology and proteome expression. The serum biomarkers showed decreasing trends in total protein, albumin and potassium concentrations and an average increase of total bilirubin and phosphate concentration towards the end of the treatment period. Principal component analysis clearly distinguished correlated pairs of biomarkers and also demonstrates a shift from acute to chronic effects as treatment progresses. Proteomic analysis confirmed alterations of proteins involved in clot formation, immune reaction and free heme binding. Tissue damage after the series of delousing treatments, exhibited increased deposits of hemosiderin. Results from this study suggest an impact of azamethiphos on trout health through intravascular haemolysis and consequently from pathophysiologic process of haemoglobin metabolism and its products, causing chronic kidney injury from iron deposits. This is the first report to demonstrate in fish the impact of active iron accumulation in different organs from physiological processes that can seriously impair normal function.
Original languageEnglish
Article number16217
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 7 Nov 2019

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Phthiraptera
Aquaculture
Oncorhynchus mykiss
Oceans and Seas
Health Status
Iron
Biomarkers
Physiological Phenomena
Salmonidae
Hemosiderin
Clinical Chemistry
Trout
Proteome
Hemolysis
Principal Component Analysis
Heme
Proteomics
Albumins
Potassium
Fishes

Keywords

  • Salmo-salar
  • Emamectin benzoate
  • Atlantic salmon
  • Oxidative stress
  • In-vivo
  • Fish
  • Blood
  • Tissues
  • Deltamethrin
  • Insecticides

Cite this

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title = "Cumulative impact of anti-sea lice treatment (azamethiphos) on health status of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) in aquaculture",
abstract = "Despite its widespread use in aquaculture, the impact of chemical anti-sea lice treatment on salmonids following application in a commercial farm has not been previously reported. This work reports the cumulative effect of three consecutive anti-sea lice treatments using azamethiphos on the health status of aquaculture reared rainbow trout through the investigation of clinical chemistry, histopathology and proteome expression. The serum biomarkers showed decreasing trends in total protein, albumin and potassium concentrations and an average increase of total bilirubin and phosphate concentration towards the end of the treatment period. Principal component analysis clearly distinguished correlated pairs of biomarkers and also demonstrates a shift from acute to chronic effects as treatment progresses. Proteomic analysis confirmed alterations of proteins involved in clot formation, immune reaction and free heme binding. Tissue damage after the series of delousing treatments, exhibited increased deposits of hemosiderin. Results from this study suggest an impact of azamethiphos on trout health through intravascular haemolysis and consequently from pathophysiologic process of haemoglobin metabolism and its products, causing chronic kidney injury from iron deposits. This is the first report to demonstrate in fish the impact of active iron accumulation in different organs from physiological processes that can seriously impair normal function.",
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Cumulative impact of anti-sea lice treatment (azamethiphos) on health status of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) in aquaculture. / Barisic, Josip; Cannon, Stuart; Quinn, Brian.

In: Scientific Reports, Vol. 9, 16217, 07.11.2019.

Research output: Contribution to journalArticle

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