Creatine supplementation and VO₂max: a systematic review and meta-analysis

Although creatine supplementation is well-known to increase exercise performance in acute high-intensity exercises, its role in aerobic performance based on VO₂max is more controversial. Thus, we performed a systematic review and meta-analysis on the effects of creatine supplementation on VO₂max. PubMed, Cochrane, Embase, and ScienceDirect were searched for randomized controlled trials (RCTs) reporting VO₂max in creatine supplementation and placebo groups before and after supplementation. We computed a random-effects meta-analysis on VO₂max at baseline, within groups following supplementation, on changes on VO₂max between groups, and after supplementation between groups. Sensitivity analyses and meta-regression were conducted. We included 19 RCTs for a total of 424 individuals (mean age 30 years old, 82% men). VO₂max did not differ at baseline between groups (creatine and placebo). Participants in both groups were engaged in exercise interventions in most studies (80%). Using changes in VO₂max, VO₂max increased in both groups but increased less after creatine supplementation than placebo (effect size [ES] = −0.32, 95%CI = −0.51 to −0.12, p = 0.002). Comparisons after creatine supplementation confirmed a lower VO₂max in the creatine group compared to the placebo group (ES= −0.20, 95%CI = −0.39 to −0.001, p = 0.049). Meta-analysis after exclusion from meta-funnel resulted in similar outcomes in a subgroup of young and healthy participants. Meta-regressions on characteristics of supplementation, physical training, or sociodemographic were not statistically significant. Creatine supplementation has a negative effect on VO₂max, regardless of the characteristics of training, supplementation, or population characteristics.