Controllable stabilization of Poly(N-isopropylacrylamide)-based Microgel Films through biomimetic mineralization of Calcium Carbonate

Yongqing Xia, Yanfeng Gu, Xuan Zhou, Hai Xu, Xiubo Zhao, Mohammed Yaseen, Jian Ren Lu

Research output: Contribution to journalArticle

Abstract

Two types of thermoresponsive microgels, poly(N-isopropylacrylamide) (PNIPAM) microgels and poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAMAC) microgels were synthesized and used as templates for the mineralization of amorphous calcium carbonate (ACC) by diffusion of CO2 vapor under ambient conditions. Thermosensitive PNIPAM/CaCO3 hybrid macroscopic hydrogels and micrometer sized PNIPAMAC/CaCO3 hybrid microgels were controllably obtained and different mineralization mechanistic processes were proposed The impact of the loaded CaCO3 on the size, morphology, stability, and thermosensitivity of the microgels was also analyzed. PNIPAM/CaCO3 hybrid macrogels had a slight decrease in thermoresponsive phase transition temperature, while PNIPAMAC/CaCO3 hybrid microgels showed a clear increase in phase transition temperature. The difference reflected different amount and location of ACC in the gel network, causing different interactions with polymer chains. The PNIPAMAC/CaCO3 microgels formed stable monolayer films on bare silica wafers and glass coverslips upon drying. The microgel films could facilitate the attachment and growth of 3T3 fibroblast. cells and their subsequent detachment upon temperature drop from 37 degrees C to the ambient condition around 20 degrees C, thus, offering a convenient procedure for cell harvesting.
Original languageEnglish
Pages (from-to)2299-2308
Number of pages10
JournalBiomacromolecules
Volume13
Issue number8
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

Cite this

Xia, Yongqing ; Gu, Yanfeng ; Zhou, Xuan ; Xu, Hai ; Zhao, Xiubo ; Yaseen, Mohammed ; Lu, Jian Ren. / Controllable stabilization of Poly(N-isopropylacrylamide)-based Microgel Films through biomimetic mineralization of Calcium Carbonate. In: Biomacromolecules. 2012 ; Vol. 13, No. 8. pp. 2299-2308.
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title = "Controllable stabilization of Poly(N-isopropylacrylamide)-based Microgel Films through biomimetic mineralization of Calcium Carbonate",
abstract = "Two types of thermoresponsive microgels, poly(N-isopropylacrylamide) (PNIPAM) microgels and poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAMAC) microgels were synthesized and used as templates for the mineralization of amorphous calcium carbonate (ACC) by diffusion of CO2 vapor under ambient conditions. Thermosensitive PNIPAM/CaCO3 hybrid macroscopic hydrogels and micrometer sized PNIPAMAC/CaCO3 hybrid microgels were controllably obtained and different mineralization mechanistic processes were proposed The impact of the loaded CaCO3 on the size, morphology, stability, and thermosensitivity of the microgels was also analyzed. PNIPAM/CaCO3 hybrid macrogels had a slight decrease in thermoresponsive phase transition temperature, while PNIPAMAC/CaCO3 hybrid microgels showed a clear increase in phase transition temperature. The difference reflected different amount and location of ACC in the gel network, causing different interactions with polymer chains. The PNIPAMAC/CaCO3 microgels formed stable monolayer films on bare silica wafers and glass coverslips upon drying. The microgel films could facilitate the attachment and growth of 3T3 fibroblast. cells and their subsequent detachment upon temperature drop from 37 degrees C to the ambient condition around 20 degrees C, thus, offering a convenient procedure for cell harvesting.",
author = "Yongqing Xia and Yanfeng Gu and Xuan Zhou and Hai Xu and Xiubo Zhao and Mohammed Yaseen and Lu, {Jian Ren}",
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Controllable stabilization of Poly(N-isopropylacrylamide)-based Microgel Films through biomimetic mineralization of Calcium Carbonate. / Xia, Yongqing; Gu, Yanfeng; Zhou, Xuan; Xu, Hai; Zhao, Xiubo; Yaseen, Mohammed; Lu, Jian Ren.

In: Biomacromolecules, Vol. 13, No. 8, 08.2012, p. 2299-2308.

Research output: Contribution to journalArticle

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N2 - Two types of thermoresponsive microgels, poly(N-isopropylacrylamide) (PNIPAM) microgels and poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAMAC) microgels were synthesized and used as templates for the mineralization of amorphous calcium carbonate (ACC) by diffusion of CO2 vapor under ambient conditions. Thermosensitive PNIPAM/CaCO3 hybrid macroscopic hydrogels and micrometer sized PNIPAMAC/CaCO3 hybrid microgels were controllably obtained and different mineralization mechanistic processes were proposed The impact of the loaded CaCO3 on the size, morphology, stability, and thermosensitivity of the microgels was also analyzed. PNIPAM/CaCO3 hybrid macrogels had a slight decrease in thermoresponsive phase transition temperature, while PNIPAMAC/CaCO3 hybrid microgels showed a clear increase in phase transition temperature. The difference reflected different amount and location of ACC in the gel network, causing different interactions with polymer chains. The PNIPAMAC/CaCO3 microgels formed stable monolayer films on bare silica wafers and glass coverslips upon drying. The microgel films could facilitate the attachment and growth of 3T3 fibroblast. cells and their subsequent detachment upon temperature drop from 37 degrees C to the ambient condition around 20 degrees C, thus, offering a convenient procedure for cell harvesting.

AB - Two types of thermoresponsive microgels, poly(N-isopropylacrylamide) (PNIPAM) microgels and poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAMAC) microgels were synthesized and used as templates for the mineralization of amorphous calcium carbonate (ACC) by diffusion of CO2 vapor under ambient conditions. Thermosensitive PNIPAM/CaCO3 hybrid macroscopic hydrogels and micrometer sized PNIPAMAC/CaCO3 hybrid microgels were controllably obtained and different mineralization mechanistic processes were proposed The impact of the loaded CaCO3 on the size, morphology, stability, and thermosensitivity of the microgels was also analyzed. PNIPAM/CaCO3 hybrid macrogels had a slight decrease in thermoresponsive phase transition temperature, while PNIPAMAC/CaCO3 hybrid microgels showed a clear increase in phase transition temperature. The difference reflected different amount and location of ACC in the gel network, causing different interactions with polymer chains. The PNIPAMAC/CaCO3 microgels formed stable monolayer films on bare silica wafers and glass coverslips upon drying. The microgel films could facilitate the attachment and growth of 3T3 fibroblast. cells and their subsequent detachment upon temperature drop from 37 degrees C to the ambient condition around 20 degrees C, thus, offering a convenient procedure for cell harvesting.

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