Conditional and reversible disruption of essential herpesvirus proteins.

Mandy Glass; Andreas  Busche; Karen Wagner; Martin Messerle; Eva-Maria Borst

doi:10.1038/nmeth.1346

Conditional and reversible disruption of essential herpesvirus proteins.

Mandy Glass, Andreas Busche, Karen Wagner, Martin Messerle, Eva-Maria Borst

Research output: Contribution to journal › Letter › peer-review

48 Citations (Scopus)

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

Original language	English
Pages (from-to)	577-579
Number of pages	3
Journal	Nature Methods
Volume	6
Issue number	8
DOIs	https://doi.org/10.1038/nmeth.1346
Publication status	Published - Aug 2009
Externally published	Yes

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10.1038/nmeth.1346

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title = "Conditional and reversible disruption of essential herpesvirus proteins.",

abstract = "Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.",

author = "Mandy Glass and Andreas Busche and Karen Wagner and Martin Messerle and Eva-Maria Borst",

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AU - Glass, Mandy

AU - Busche, Andreas

AU - Wagner, Karen

AU - Messerle, Martin

AU - Borst, Eva-Maria

PY - 2009/8

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N2 - Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

AB - Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

U2 - 10.1038/nmeth.1346

DO - 10.1038/nmeth.1346

M3 - Letter

SN - 1548-7105

VL - 6

SP - 577

EP - 579

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

Conditional and reversible disruption of essential herpesvirus proteins.

Abstract

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