Conditional and reversible disruption of essential herpesvirus proteins.

Mandy Glass, Andreas Busche, Karen Wagner, Martin Messerle, Eva-Maria Borst

Research output: Contribution to journalLetterpeer-review

41 Citations (Scopus)

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Original languageEnglish
Pages (from-to)577-579
Number of pages3
JournalNature Methods
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

Fingerprint

Dive into the research topics of 'Conditional and reversible disruption of essential herpesvirus proteins.'. Together they form a unique fingerprint.

Cite this