Conditional and reversible disruption of essential herpesvirus proteins.

Mandy Glass, Andreas Busche, Karen Wagner, Martin Messerle, Eva-Maria Borst

Research output: Contribution to journalLetterpeer-review

45 Citations (Scopus)


Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Original languageEnglish
Pages (from-to)577-579
Number of pages3
JournalNature Methods
Issue number8
Publication statusPublished - Aug 2009
Externally publishedYes


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