Conditional and reversible disruption of essential herpesvirus proteins.

Mandy Glass, Andreas Busche, Karen Wagner, Martin Messerle, Eva-Maria Borst

Research output: Contribution to journalLetter

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.
Original languageEnglish
Pages (from-to)577-579
Number of pages3
JournalNature Methods
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

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Herpesviridae
Viruses
Viral Fusion Proteins
Tacrolimus Binding Proteins
Proteins
Cytomegalovirus
Ligands
Growth
Genes

Cite this

Glass, Mandy ; Busche, Andreas ; Wagner, Karen ; Messerle, Martin ; Borst, Eva-Maria. / Conditional and reversible disruption of essential herpesvirus proteins. In: Nature Methods. 2009 ; Vol. 6, No. 8. pp. 577-579.
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Glass, M, Busche, A, Wagner, K, Messerle, M & Borst, E-M 2009, 'Conditional and reversible disruption of essential herpesvirus proteins.' Nature Methods, vol. 6, no. 8, pp. 577-579. https://doi.org/10.1038/nmeth.1346

Conditional and reversible disruption of essential herpesvirus proteins. / Glass, Mandy; Busche, Andreas; Wagner, Karen; Messerle, Martin; Borst, Eva-Maria.

In: Nature Methods, Vol. 6, No. 8, 08.2009, p. 577-579.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Conditional and reversible disruption of essential herpesvirus proteins.

AU - Glass, Mandy

AU - Busche, Andreas

AU - Wagner, Karen

AU - Messerle, Martin

AU - Borst, Eva-Maria

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AB - Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

U2 - 10.1038/nmeth.1346

DO - 10.1038/nmeth.1346

M3 - Letter

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SP - 577

EP - 579

JO - Nature Methods

JF - Nature Methods

SN - 1548-7105

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ER -