Comparative study of physiological changes in turbot Scophthalmus maximus in different living conditions

Silvia Križanac; Natalija Topić Popović; Josip Barišić; Blanka Beer-Ljubić; Maro Bujak; Sanja Babić; Krunoslav Bojanić; Rozelindra Čož-Rakovac; Daniel Matulić; Ivančica Strunjak-Perović

doi:10.3390/app12094201

Comparative study of physiological changes in turbot Scophthalmus maximus in different living conditions

Silvia Križanac
, Natalija Topić Popović
, Josip Barišić
, Blanka Beer-Ljubić
, Maro Bujak
, Sanja Babić
, Krunoslav Bojanić
, Rozelindra Čož-Rakovac
, Daniel Matulić
, Ivančica Strunjak-Perović^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

40 Downloads (Pure)

Abstract

The aim of this study was to compare the organismal responses of farmed and wild-caught turbot, Scophthalmus maximus, based on analyses of biochemical plasma parameters, leukocyte profile, and histological tissue profile of gills, kidney, liver, intestine, and spleen, as well as gene expression of stress proteins in kidney and liver tissue. The results revealed significant differences in plasma triglycerides (TRIG), total protein (TP), albumin (ALB), globulin (GLOB), bilirubin (TBIL), creatinine (CRE) levels, creatine kinase (CK), and superoxide dismutase (SOD) activities that were higher, and A/G ratio, calcium (Ca) and phosphorus (P) concentrations, alkaline phosphatase (ALP), and glutathione peroxidase (GPx) activity, which were lower in farmed population. The neutrophil-leukocyte (N:L) ratio and gene expression of HSP70, HSP90, and WAP65-2 were increased in the wild-caught turbot. The wild-caught turbot were infested with the gill digenean parasite Dactylogyrus sp. and tapeworm Bothriocephalus scorpii. The obtained results provide valuable data for the assessment of the physiological responses of turbot for future comparative studies of the effects of various endogenous and exogenous factors on homeostasis of this species.

Original language	English
Article number	4201
Number of pages	12
Journal	Applied Sciences (Switzerland)
Volume	12
Issue number	9
DOIs	https://doi.org/10.3390/app12094201
Publication status	Published - 21 Apr 2022

Keywords

Adriatic Sea
blood biochemistry
heat shock proteins
histology
leukocyte
oxidative stress

Access to Document

10.3390/app12094201Licence: CC BY 4.0

2022 04 19 Krizanac et al Comparative finalFinal published version, 2.52 MBLicence: CC BY 4.0

Cite this

@article{1de151c37ac14c43902bc853a1c0a85f,

title = "Comparative study of physiological changes in turbot Scophthalmus maximus in different living conditions",

abstract = "The aim of this study was to compare the organismal responses of farmed and wild-caught turbot, Scophthalmus maximus, based on analyses of biochemical plasma parameters, leukocyte profile, and histological tissue profile of gills, kidney, liver, intestine, and spleen, as well as gene expression of stress proteins in kidney and liver tissue. The results revealed significant differences in plasma triglycerides (TRIG), total protein (TP), albumin (ALB), globulin (GLOB), bilirubin (TBIL), creatinine (CRE) levels, creatine kinase (CK), and superoxide dismutase (SOD) activities that were higher, and A/G ratio, calcium (Ca) and phosphorus (P) concentrations, alkaline phosphatase (ALP), and glutathione peroxidase (GPx) activity, which were lower in farmed population. The neutrophil-leukocyte (N:L) ratio and gene expression of HSP70, HSP90, and WAP65-2 were increased in the wild-caught turbot. The wild-caught turbot were infested with the gill digenean parasite Dactylogyrus sp. and tapeworm Bothriocephalus scorpii. The obtained results provide valuable data for the assessment of the physiological responses of turbot for future comparative studies of the effects of various endogenous and exogenous factors on homeostasis of this species.",

keywords = "Adriatic Sea, blood biochemistry, heat shock proteins, histology, leukocyte, oxidative stress",

author = "Silvia Kri{\v z}anac and Popovi{\'c}, \{Natalija Topi{\'c}\} and Josip Bari{\v s}i{\'c} and Blanka Beer-Ljubi{\'c} and Maro Bujak and Sanja Babi{\'c} and Krunoslav Bojani{\'c} and Rozelindra {\v C}o{\v z}-Rakovac and Daniel Matuli{\'c} and Ivan{\v c}ica Strunjak-Perovi{\'c}",

year = "2022",

month = apr,

day = "21",

doi = "10.3390/app12094201",

language = "English",

volume = "12",

journal = "Applied Sciences (Switzerland)",

issn = "2076-3417",

publisher = "Multidisciplinary Digital Publishing Institute",

number = "9",

}

TY - JOUR

T1 - Comparative study of physiological changes in turbot Scophthalmus maximus in different living conditions

AU - Križanac, Silvia

AU - Popović, Natalija Topić

AU - Barišić, Josip

AU - Beer-Ljubić, Blanka

AU - Bujak, Maro

AU - Babić, Sanja

AU - Bojanić, Krunoslav

AU - Čož-Rakovac, Rozelindra

AU - Matulić, Daniel

AU - Strunjak-Perović, Ivančica

PY - 2022/4/21

Y1 - 2022/4/21

N2 - The aim of this study was to compare the organismal responses of farmed and wild-caught turbot, Scophthalmus maximus, based on analyses of biochemical plasma parameters, leukocyte profile, and histological tissue profile of gills, kidney, liver, intestine, and spleen, as well as gene expression of stress proteins in kidney and liver tissue. The results revealed significant differences in plasma triglycerides (TRIG), total protein (TP), albumin (ALB), globulin (GLOB), bilirubin (TBIL), creatinine (CRE) levels, creatine kinase (CK), and superoxide dismutase (SOD) activities that were higher, and A/G ratio, calcium (Ca) and phosphorus (P) concentrations, alkaline phosphatase (ALP), and glutathione peroxidase (GPx) activity, which were lower in farmed population. The neutrophil-leukocyte (N:L) ratio and gene expression of HSP70, HSP90, and WAP65-2 were increased in the wild-caught turbot. The wild-caught turbot were infested with the gill digenean parasite Dactylogyrus sp. and tapeworm Bothriocephalus scorpii. The obtained results provide valuable data for the assessment of the physiological responses of turbot for future comparative studies of the effects of various endogenous and exogenous factors on homeostasis of this species.

AB - The aim of this study was to compare the organismal responses of farmed and wild-caught turbot, Scophthalmus maximus, based on analyses of biochemical plasma parameters, leukocyte profile, and histological tissue profile of gills, kidney, liver, intestine, and spleen, as well as gene expression of stress proteins in kidney and liver tissue. The results revealed significant differences in plasma triglycerides (TRIG), total protein (TP), albumin (ALB), globulin (GLOB), bilirubin (TBIL), creatinine (CRE) levels, creatine kinase (CK), and superoxide dismutase (SOD) activities that were higher, and A/G ratio, calcium (Ca) and phosphorus (P) concentrations, alkaline phosphatase (ALP), and glutathione peroxidase (GPx) activity, which were lower in farmed population. The neutrophil-leukocyte (N:L) ratio and gene expression of HSP70, HSP90, and WAP65-2 were increased in the wild-caught turbot. The wild-caught turbot were infested with the gill digenean parasite Dactylogyrus sp. and tapeworm Bothriocephalus scorpii. The obtained results provide valuable data for the assessment of the physiological responses of turbot for future comparative studies of the effects of various endogenous and exogenous factors on homeostasis of this species.

KW - Adriatic Sea

KW - blood biochemistry

KW - heat shock proteins

KW - histology

KW - leukocyte

KW - oxidative stress

UR - https://www.scopus.com/pages/publications/85129132115

U2 - 10.3390/app12094201

DO - 10.3390/app12094201

M3 - Article

AN - SCOPUS:85129132115

SN - 2076-3417

VL - 12

JO - Applied Sciences (Switzerland)

JF - Applied Sciences (Switzerland)

IS - 9

M1 - 4201

ER -

Comparative study of physiological changes in turbot Scophthalmus maximus in different living conditions

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this