Chemical profiling and biological screening of some River Nile derived-microorganisms

Momen M. Lotfy, Hossam M. Hassan, Rabab Mohammed, Mona Hetta, Ahmed O. El-Gendy, Mostafa E. Rateb, Mohamed A. Zaki, Noha M. Gamaleldin

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Abstract

Aims

Chemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antileishmanial, antitrypanosomal, and antimalarial activities were investigated.

Methods

Identification of the microbial isolates were carried out using bacterial 16S rRNA and fungal 18S rRNA gene sequencing. Chemical profiling of the EtOAc extracts using LC-HRESIMS spectroscopy was carried out. The in vitro antimicrobial screening using the modified version of the CLSI method, antileishmanial and antitrypanosomal activities were screened using Leishmania donovani promastigote assay, L. donovani axenic amastigote assay, Trypanosoma brucei trypamastigotes assay and THP1 toxicity assay. The in vitro antimalarial activities against D6 (chloroquine sensitive) and W2 (chloroquine-resistant) strains of Plasmodium falciparum were evaluated.

Results

Seven isolated microorganisms were identified as Streptomyces indiaensis, Bacillus safensis, B. anthracis, Bacillus sp., and Aspergillus awamori. Chemical investigation of different extracts showed several bioactive compounds, identified as; nigragillin, 5-caboxybenzofuran and dyramide B from A. awamori and actinopolysporin B from S. indiaensis. On the other hand many nitrogenous compounds with high molecular weights showed no hits that may correspond to new long chain and/or cyclic peptides. The EtOAc extract of B. safensis fermentation broth showed the highest activity against P. falciparum D6 and P. falciparum W2 (IC50 = 25.94 and 27.28 μg/mL, respectively), while two isolates S. indiaensis and Bacillus sp. RN-011 extracts showed the highest antitrypanosomal activity (IC50 = 0.8 and 0.96 μg/mL).

Conclusion

The River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated.

Original languageEnglish
Article number787
Number of pages10
JournalFrontiers in Microbiology
Volume10
Early online date12 Apr 2019
DOIs
Publication statusE-pub ahead of print - 12 Apr 2019

Fingerprint

Plasmodium falciparum
Rivers
Leishmania donovani
Chloroquine
Antimalarials
Bacillus
Inhibitory Concentration 50
Antiparasitic Agents
Bacillus anthracis
Cyclic Peptides
Trypanosoma brucei brucei
Streptomyces
Aspergillus
rRNA Genes
Fermentation
Ecosystem
Spectrum Analysis
Molecular Weight
In Vitro Techniques

Keywords

  • chemical profiling
  • antimicrobial
  • antileishmanial
  • antitrypanosomal
  • antimalarial

Cite this

Lotfy, M. M., Hassan, H. M., Mohammed, R., Hetta, M., El-Gendy, A. O., Rateb, M. E., ... Gamaleldin, N. M. (2019). Chemical profiling and biological screening of some River Nile derived-microorganisms. Frontiers in Microbiology, 10, [787]. https://doi.org/10.3389/fmicb.2019.00787
Lotfy, Momen M. ; Hassan, Hossam M. ; Mohammed, Rabab ; Hetta, Mona ; El-Gendy, Ahmed O. ; Rateb, Mostafa E. ; Zaki, Mohamed A. ; Gamaleldin, Noha M. / Chemical profiling and biological screening of some River Nile derived-microorganisms. In: Frontiers in Microbiology. 2019 ; Vol. 10.
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Lotfy, MM, Hassan, HM, Mohammed, R, Hetta, M, El-Gendy, AO, Rateb, ME, Zaki, MA & Gamaleldin, NM 2019, 'Chemical profiling and biological screening of some River Nile derived-microorganisms' Frontiers in Microbiology, vol. 10, 787. https://doi.org/10.3389/fmicb.2019.00787

Chemical profiling and biological screening of some River Nile derived-microorganisms. / Lotfy, Momen M.; Hassan, Hossam M.; Mohammed, Rabab; Hetta, Mona; El-Gendy, Ahmed O.; Rateb, Mostafa E.; Zaki, Mohamed A.; Gamaleldin, Noha M.

In: Frontiers in Microbiology, Vol. 10, 787, 12.04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemical profiling and biological screening of some River Nile derived-microorganisms

AU - Lotfy, Momen M.

AU - Hassan, Hossam M.

AU - Mohammed, Rabab

AU - Hetta, Mona

AU - El-Gendy, Ahmed O.

AU - Rateb, Mostafa E.

AU - Zaki, Mohamed A.

AU - Gamaleldin, Noha M.

PY - 2019/4/12

Y1 - 2019/4/12

N2 - AimsChemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antileishmanial, antitrypanosomal, and antimalarial activities were investigated.MethodsIdentification of the microbial isolates were carried out using bacterial 16S rRNA and fungal 18S rRNA gene sequencing. Chemical profiling of the EtOAc extracts using LC-HRESIMS spectroscopy was carried out. The in vitro antimicrobial screening using the modified version of the CLSI method, antileishmanial and antitrypanosomal activities were screened using Leishmania donovani promastigote assay, L. donovani axenic amastigote assay, Trypanosoma brucei trypamastigotes assay and THP1 toxicity assay. The in vitro antimalarial activities against D6 (chloroquine sensitive) and W2 (chloroquine-resistant) strains of Plasmodium falciparum were evaluated.ResultsSeven isolated microorganisms were identified as Streptomyces indiaensis, Bacillus safensis, B. anthracis, Bacillus sp., and Aspergillus awamori. Chemical investigation of different extracts showed several bioactive compounds, identified as; nigragillin, 5-caboxybenzofuran and dyramide B from A. awamori and actinopolysporin B from S. indiaensis. On the other hand many nitrogenous compounds with high molecular weights showed no hits that may correspond to new long chain and/or cyclic peptides. The EtOAc extract of B. safensis fermentation broth showed the highest activity against P. falciparum D6 and P. falciparum W2 (IC50 = 25.94 and 27.28 μg/mL, respectively), while two isolates S. indiaensis and Bacillus sp. RN-011 extracts showed the highest antitrypanosomal activity (IC50 = 0.8 and 0.96 μg/mL).ConclusionThe River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated.

AB - AimsChemical and biological studies of the River Nile derived-microorganisms are limited. Hence, this work was carried out to screen the River Nile habitat. Identification of the isolated organisms, chemical profiling of their ethyl acetate extracts as well as screening of their antimicrobial, antileishmanial, antitrypanosomal, and antimalarial activities were investigated.MethodsIdentification of the microbial isolates were carried out using bacterial 16S rRNA and fungal 18S rRNA gene sequencing. Chemical profiling of the EtOAc extracts using LC-HRESIMS spectroscopy was carried out. The in vitro antimicrobial screening using the modified version of the CLSI method, antileishmanial and antitrypanosomal activities were screened using Leishmania donovani promastigote assay, L. donovani axenic amastigote assay, Trypanosoma brucei trypamastigotes assay and THP1 toxicity assay. The in vitro antimalarial activities against D6 (chloroquine sensitive) and W2 (chloroquine-resistant) strains of Plasmodium falciparum were evaluated.ResultsSeven isolated microorganisms were identified as Streptomyces indiaensis, Bacillus safensis, B. anthracis, Bacillus sp., and Aspergillus awamori. Chemical investigation of different extracts showed several bioactive compounds, identified as; nigragillin, 5-caboxybenzofuran and dyramide B from A. awamori and actinopolysporin B from S. indiaensis. On the other hand many nitrogenous compounds with high molecular weights showed no hits that may correspond to new long chain and/or cyclic peptides. The EtOAc extract of B. safensis fermentation broth showed the highest activity against P. falciparum D6 and P. falciparum W2 (IC50 = 25.94 and 27.28 μg/mL, respectively), while two isolates S. indiaensis and Bacillus sp. RN-011 extracts showed the highest antitrypanosomal activity (IC50 = 0.8 and 0.96 μg/mL).ConclusionThe River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated.

KW - chemical profiling

KW - antimicrobial

KW - antileishmanial

KW - antitrypanosomal

KW - antimalarial

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DO - 10.3389/fmicb.2019.00787

M3 - Article

VL - 10

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 787

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