Characterization of natural bioactive compounds produced by isolated bacteria from compost of aromatic plants

Nourhan O. El-Helw, Ahmed O. El-Gendy, Eman El-Gebaly, Hossam M. Hassan, Mostafa E. Rateb, Khalid A. El-Nesr

Research output: Contribution to journalArticle

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Abstract

AIMS: This study aimed to highlight the importance of compost from aromatic plants as a stunning source for several bio active compounds generated from their inhabited thermophilic bacteria. Some of the isolated compounds could have a potential role in the treatment of microbial infections.

METHODS AND RESULTS: A total of forty different thermophilic bacteria were isolated from compost samples during their thermophilic stage. These isolates were tested for their antimicrobial capabilities against different Gram-positive and negative bacteria using agar diffusion and double layer agar methods. The potential isolates were further identified based on morphological, biochemical and 16S rRNA gene sequencing methods. They were subjected to submerged state fermentation and the total crude metabolites were recovered using ethyl acetate (EtOAc) extraction. All bioactive metabolites were identified using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). It was observed that two out of forty isolates were remarkably active against Gram positive bacteria. These isolates were genetically identified as Bacillus species and their different active metabolites were characterized in the EtOAc extracts using LC-HRMS.

CONCLUSION: LC-HRMS analysis of EtOAc extracts revealed the presence of active metabolites that are responsible for antimicrobial activities.

SIGNIFICANCE AND IMPACT OF STUDY: To the best of our knowledge, this is the first time to identify bioactive antimicrobial metabolites from retrieved compost microorganisms in Egypt. So, compost could be a beneficial area for research as a reliable and continuous natural source for different uncountable communities of bacteria. This article is protected by copyright. All rights reserved.

Original languageEnglish
Pages (from-to)443-451
Number of pages9
JournalJournal of Applied Microbiology
Volume126
Issue number2
Early online date24 Aug 2018
DOIs
Publication statusPublished - 28 Feb 2019
Externally publishedYes

Fingerprint

Soil
Liquid Chromatography
Bacteria
Mass Spectrometry
Gram-Positive Bacteria
Agar
Egypt
Gram-Negative Bacteria
rRNA Genes
Bacillus
Fermentation
Infection
Research

Keywords

  • Compost
  • aromatic plants
  • thermophilic bacteria
  • bioactive natural compounds

Cite this

El-Helw, Nourhan O. ; El-Gendy, Ahmed O. ; El-Gebaly, Eman ; Hassan, Hossam M. ; Rateb, Mostafa E. ; El-Nesr, Khalid A. / Characterization of natural bioactive compounds produced by isolated bacteria from compost of aromatic plants. In: Journal of Applied Microbiology. 2019 ; Vol. 126, No. 2. pp. 443-451.
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Characterization of natural bioactive compounds produced by isolated bacteria from compost of aromatic plants. / El-Helw, Nourhan O.; El-Gendy, Ahmed O.; El-Gebaly, Eman; Hassan, Hossam M.; Rateb, Mostafa E.; El-Nesr, Khalid A.

In: Journal of Applied Microbiology, Vol. 126, No. 2, 28.02.2019, p. 443-451.

Research output: Contribution to journalArticle

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AU - El-Helw, Nourhan O.

AU - El-Gendy, Ahmed O.

AU - El-Gebaly, Eman

AU - Hassan, Hossam M.

AU - Rateb, Mostafa E.

AU - El-Nesr, Khalid A.

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N2 - AIMS: This study aimed to highlight the importance of compost from aromatic plants as a stunning source for several bio active compounds generated from their inhabited thermophilic bacteria. Some of the isolated compounds could have a potential role in the treatment of microbial infections.METHODS AND RESULTS: A total of forty different thermophilic bacteria were isolated from compost samples during their thermophilic stage. These isolates were tested for their antimicrobial capabilities against different Gram-positive and negative bacteria using agar diffusion and double layer agar methods. The potential isolates were further identified based on morphological, biochemical and 16S rRNA gene sequencing methods. They were subjected to submerged state fermentation and the total crude metabolites were recovered using ethyl acetate (EtOAc) extraction. All bioactive metabolites were identified using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). It was observed that two out of forty isolates were remarkably active against Gram positive bacteria. These isolates were genetically identified as Bacillus species and their different active metabolites were characterized in the EtOAc extracts using LC-HRMS.CONCLUSION: LC-HRMS analysis of EtOAc extracts revealed the presence of active metabolites that are responsible for antimicrobial activities.SIGNIFICANCE AND IMPACT OF STUDY: To the best of our knowledge, this is the first time to identify bioactive antimicrobial metabolites from retrieved compost microorganisms in Egypt. So, compost could be a beneficial area for research as a reliable and continuous natural source for different uncountable communities of bacteria. This article is protected by copyright. All rights reserved.

AB - AIMS: This study aimed to highlight the importance of compost from aromatic plants as a stunning source for several bio active compounds generated from their inhabited thermophilic bacteria. Some of the isolated compounds could have a potential role in the treatment of microbial infections.METHODS AND RESULTS: A total of forty different thermophilic bacteria were isolated from compost samples during their thermophilic stage. These isolates were tested for their antimicrobial capabilities against different Gram-positive and negative bacteria using agar diffusion and double layer agar methods. The potential isolates were further identified based on morphological, biochemical and 16S rRNA gene sequencing methods. They were subjected to submerged state fermentation and the total crude metabolites were recovered using ethyl acetate (EtOAc) extraction. All bioactive metabolites were identified using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). It was observed that two out of forty isolates were remarkably active against Gram positive bacteria. These isolates were genetically identified as Bacillus species and their different active metabolites were characterized in the EtOAc extracts using LC-HRMS.CONCLUSION: LC-HRMS analysis of EtOAc extracts revealed the presence of active metabolites that are responsible for antimicrobial activities.SIGNIFICANCE AND IMPACT OF STUDY: To the best of our knowledge, this is the first time to identify bioactive antimicrobial metabolites from retrieved compost microorganisms in Egypt. So, compost could be a beneficial area for research as a reliable and continuous natural source for different uncountable communities of bacteria. This article is protected by copyright. All rights reserved.

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