Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry

Kaveh Emami, Vahid Askari, Matthias Ullrich, Khwajah Mohinudeen, Arga Chandrashekar Anil, Lidita Khandeparker, J. Grant Burgess, Ehsan Mesbahi

Research output: Contribution to journalArticle

Abstract

To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
Original languageEnglish
JournalPLoS ONE
Volume7
Issue number6
DOIs
Publication statusPublished - 7 Jun 2012
Externally publishedYes

Cite this

Emami, K., Askari, V., Ullrich, M., Mohinudeen, K., Anil, A. C., Khandeparker, L., ... Mesbahi, E. (2012). Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry. PLoS ONE, 7(6). https://doi.org/10.1371/journal.pone.0038515
Emami, Kaveh ; Askari, Vahid ; Ullrich, Matthias ; Mohinudeen, Khwajah ; Anil, Arga Chandrashekar ; Khandeparker, Lidita ; Burgess, J. Grant ; Mesbahi, Ehsan. / Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry. In: PLoS ONE. 2012 ; Vol. 7, No. 6.
@article{caf923f2e8fd46b9b03cc3649794e671,
title = "Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry",
abstract = "To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.",
author = "Kaveh Emami and Vahid Askari and Matthias Ullrich and Khwajah Mohinudeen and Anil, {Arga Chandrashekar} and Lidita Khandeparker and Burgess, {J. Grant} and Ehsan Mesbahi",
year = "2012",
month = "6",
day = "7",
doi = "10.1371/journal.pone.0038515",
language = "English",
volume = "7",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

Emami, K, Askari, V, Ullrich, M, Mohinudeen, K, Anil, AC, Khandeparker, L, Burgess, JG & Mesbahi, E 2012, 'Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry' PLoS ONE, vol. 7, no. 6. https://doi.org/10.1371/journal.pone.0038515

Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry. / Emami, Kaveh; Askari, Vahid; Ullrich, Matthias; Mohinudeen, Khwajah; Anil, Arga Chandrashekar; Khandeparker, Lidita; Burgess, J. Grant; Mesbahi, Ehsan.

In: PLoS ONE, Vol. 7, No. 6, 07.06.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry

AU - Emami, Kaveh

AU - Askari, Vahid

AU - Ullrich, Matthias

AU - Mohinudeen, Khwajah

AU - Anil, Arga Chandrashekar

AU - Khandeparker, Lidita

AU - Burgess, J. Grant

AU - Mesbahi, Ehsan

PY - 2012/6/7

Y1 - 2012/6/7

N2 - To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.

AB - To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.

U2 - 10.1371/journal.pone.0038515

DO - 10.1371/journal.pone.0038515

M3 - Article

VL - 7

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

ER -

Emami K, Askari V, Ullrich M, Mohinudeen K, Anil AC, Khandeparker L et al. Characterization of bacteria in ballast water Using MALDI-TOF mass spectrometry. PLoS ONE. 2012 Jun 7;7(6). https://doi.org/10.1371/journal.pone.0038515