Changes in body posture alter plasma nitrite but not nitrate concentration in humans

Luke Liddle, Christopher Monaghan, Mia Burleigh, Luke McIlvenna, David Muggeridge, Chris Easton

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Abstract

PURPOSE: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]. 
METHODS: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ~31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method. 
RESULTS: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P<0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P<0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P<0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P=0.02). Plasma [NO3-] was not different between measurement points in either condition (P>0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05). 
CONCLUSIONS: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.
Original languageEnglish
Pages (from-to)59-65
JournalNitric Oxide
Volume72
Early online date2 Dec 2017
DOIs
Publication statusPublished - 30 Jan 2018

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Nitrites
Posture
Nitrates
Plasma Volume
Plasmas
Anethum graveolens
Luminescence
Healthy Volunteers
Gases
Research Personnel
Exercise
Chemiluminescence

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@article{65792e45e16c4ca480ea3c4cea695a49,
title = "Changes in body posture alter plasma nitrite but not nitrate concentration in humans",
abstract = "PURPOSE: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]. METHODS: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ~31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method. RESULTS: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P<0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P<0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P<0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P=0.02). Plasma [NO3-] was not different between measurement points in either condition (P>0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05). CONCLUSIONS: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.",
author = "Luke Liddle and Christopher Monaghan and Mia Burleigh and Luke McIlvenna and David Muggeridge and Chris Easton",
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language = "English",
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journal = "Nitric Oxide",
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Changes in body posture alter plasma nitrite but not nitrate concentration in humans. / Liddle, Luke; Monaghan, Christopher; Burleigh, Mia ; McIlvenna, Luke; Muggeridge, David; Easton, Chris.

In: Nitric Oxide, Vol. 72, 30.01.2018, p. 59-65.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in body posture alter plasma nitrite but not nitrate concentration in humans

AU - Liddle, Luke

AU - Monaghan, Christopher

AU - Burleigh, Mia

AU - McIlvenna, Luke

AU - Muggeridge, David

AU - Easton, Chris

PY - 2018/1/30

Y1 - 2018/1/30

N2 - PURPOSE: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]. METHODS: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ~31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method. RESULTS: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P<0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P<0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P<0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P=0.02). Plasma [NO3-] was not different between measurement points in either condition (P>0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05). CONCLUSIONS: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.

AB - PURPOSE: This study evaluated the change (Δ) in plasma volume (PV), nitrate [NO3-], and nitrite [NO2-] concentration following changes in posture in the presence and absence of elevated plasma [NO3-] and [NO2-]. METHODS: Fourteen healthy participants completed two trials that were preceded by either supplementation with NO3--rich beetroot juice (BR; total of ~31 mmol NO3-) or no supplementation (CON). Both trials comprised 30 min of lying supine followed by 2 min of standing, 2 min of sitting and 5 min of sub-maximal cycling. Measurements of plasma [NO3-] and [NO2-] were made by gas-phase chemiluminescence and ΔPV was estimated using the Dill and Costill method. RESULTS: Plasma [NO2-] decreased from baseline (CON: 120 ± 49 nM, BR: 357 ± 129 nM) after lying supine for 30 min (CON 77 ± 30 nM; BR 231 ± 92 nM, both P<0.01) before increasing during standing (CON 109 ± 42 nM; BR 297 ± 105 nM, both P<0.01) and sitting (CON 131 ± 43 nM; BR 385 ± 125 nM, both P<0.01). Plasma [NO2-] remained elevated following exercise only in CON (125 ± 61 nM P=0.02). Plasma [NO3-] was not different between measurement points in either condition (P>0.05). PV increased from baseline during the supine phase before decreasing upon standing, sitting, and exercise in both trials (all P<0.05). CONCLUSIONS: Changing body posture causes rapid and consistent alterations in plasma [NO2-]. Researchers should therefore carefully consider the effect of posture when measuring this variable.

U2 - 10.1016/j.niox.2017.11.008

DO - 10.1016/j.niox.2017.11.008

M3 - Article

VL - 72

SP - 59

EP - 65

JO - Nitric Oxide

JF - Nitric Oxide

SN - 1089-8603

ER -