Cell interactions at the nanoscale: piezoelectric stimulation

Adam S G Curtis, Stuart Reid, Iain Martin, Ramanathan Vaidyanathan, Carol-Anne Smith, Habib Nikukar, Matthew J Dalby

Research output: Contribution to journalArticle

Abstract

Nanometric movements of the substrate on which endothelial cells are growing, driven by periodic sinusoidal vibration from 1 Hz to 50 Hz applied by piezo actuators, upregulate endothelin-1 and Kruppel-like factor 2 expression, and increase cell adhesion. These movements are in the z (vertical) axis and ranges from 5 to 50 nm and are similar in vertical extent to protrusions from the cells themselves already reported in the literature. White noise vibrations do not to produce these effects. Vibrational sweeps, if suitably confined within a narrow frequency range, produce similar stimulatory effects but not at wider sweeps. These effects suggest that coherent vibration is crucial for driving these cellular responses. In addition to this, the applied stimulations are observed to be close to or below the random seismic noise of the surroundings, which may suggest stochastic resonance is being employed. The stimulations also interact with the effects of nanometric patterning of the substrates on cell adhesion and Kruppel-like factor 2 and endothelin-1 expression thus linking cell reactions to nanotopographically patterned surfaces with those to mechanical stimulation.

Original languageEnglish
Pages (from-to)247-54
Number of pages8
JournalIEEE Transactions on Nanobioscience
Volume12
Issue number3
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Kruppel-Like Transcription Factors
Cell adhesion
Endothelin-1
Vibration
Cell Communication
Cell Adhesion
Endothelial cells
Substrates
White noise
Actuators
Noise
Up-Regulation
Endothelial Cells

Keywords

  • Animals
  • Cell Adhesion
  • Cell Line
  • Electric Stimulation
  • Endothelin-1
  • Kruppel-Like Transcription Factors
  • Mice
  • NF-kappa B
  • Nanostructures
  • Nanotechnology
  • Transducers

Cite this

Curtis, A. S. G., Reid, S., Martin, I., Vaidyanathan, R., Smith, C-A., Nikukar, H., & Dalby, M. J. (2013). Cell interactions at the nanoscale: piezoelectric stimulation. IEEE Transactions on Nanobioscience, 12(3), 247-54. https://doi.org/10.1109/TNB.2013.2257837
Curtis, Adam S G ; Reid, Stuart ; Martin, Iain ; Vaidyanathan, Ramanathan ; Smith, Carol-Anne ; Nikukar, Habib ; Dalby, Matthew J. / Cell interactions at the nanoscale : piezoelectric stimulation. In: IEEE Transactions on Nanobioscience. 2013 ; Vol. 12, No. 3. pp. 247-54.
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Curtis, ASG, Reid, S, Martin, I, Vaidyanathan, R, Smith, C-A, Nikukar, H & Dalby, MJ 2013, 'Cell interactions at the nanoscale: piezoelectric stimulation' IEEE Transactions on Nanobioscience, vol. 12, no. 3, pp. 247-54. https://doi.org/10.1109/TNB.2013.2257837

Cell interactions at the nanoscale : piezoelectric stimulation. / Curtis, Adam S G; Reid, Stuart; Martin, Iain; Vaidyanathan, Ramanathan; Smith, Carol-Anne; Nikukar, Habib; Dalby, Matthew J.

In: IEEE Transactions on Nanobioscience, Vol. 12, No. 3, 09.2013, p. 247-54.

Research output: Contribution to journalArticle

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T2 - piezoelectric stimulation

AU - Curtis, Adam S G

AU - Reid, Stuart

AU - Martin, Iain

AU - Vaidyanathan, Ramanathan

AU - Smith, Carol-Anne

AU - Nikukar, Habib

AU - Dalby, Matthew J

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N2 - Nanometric movements of the substrate on which endothelial cells are growing, driven by periodic sinusoidal vibration from 1 Hz to 50 Hz applied by piezo actuators, upregulate endothelin-1 and Kruppel-like factor 2 expression, and increase cell adhesion. These movements are in the z (vertical) axis and ranges from 5 to 50 nm and are similar in vertical extent to protrusions from the cells themselves already reported in the literature. White noise vibrations do not to produce these effects. Vibrational sweeps, if suitably confined within a narrow frequency range, produce similar stimulatory effects but not at wider sweeps. These effects suggest that coherent vibration is crucial for driving these cellular responses. In addition to this, the applied stimulations are observed to be close to or below the random seismic noise of the surroundings, which may suggest stochastic resonance is being employed. The stimulations also interact with the effects of nanometric patterning of the substrates on cell adhesion and Kruppel-like factor 2 and endothelin-1 expression thus linking cell reactions to nanotopographically patterned surfaces with those to mechanical stimulation.

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Curtis ASG, Reid S, Martin I, Vaidyanathan R, Smith C-A, Nikukar H et al. Cell interactions at the nanoscale: piezoelectric stimulation. IEEE Transactions on Nanobioscience. 2013 Sep;12(3):247-54. https://doi.org/10.1109/TNB.2013.2257837