Cardiopreventive effect of ethanolic extract of Date Palm Pollen against isoproterenol induced myocardial infarction in rats through the inhibition of the angiotensin-converting enzyme

Amal Daoud, Fedia Ben Mefteh, Kais Mnafgui, Mouna Turki, Salwa Jmal, Rawdha Ben Amar, Fatma Ayadi, Abdelfattah ElFeki, Leila Abid, Mostafa E. Rateb, Lassaad Belbahri, Adel Kadri, Neji Gharsallah

Research output: Contribution to journalArticle

9 Citations (Scopus)
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Abstract

The present study aimed to examine the putative preventive effect of the ethanolic extract Date Palm Pollen (DPP, Phoenix dactylifera L., family Arecaceae) on isoproterenol-induced myocardial infarction (MI) in rats. Twenty four rats were randomly divided into four groups including control. They were treated with DPP extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th and 7th day). Administration of isoproterenol exhibited indicative changes in the ECG pattern evidenced by significant elevation of ST-segment and cardiac injury markers viz.; troponin-T, creatine phosphokinase (CPK), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) by 315%, 71%, 64% and 170%, respectively as compared to control. Additionally, the angiotensin-converting enzyme (ACE) activity in plasma was increased by 33% associated to histological myocardial necrosis. However, pre-co-treatment with DPP extract improved the cardiac biomarkers injury, normalized cardiac function indices and prevented the ventricular remodeling process through inhibition of ACE activity by 34% and the inhibition of the generation of radical oxygen species. Extensive characterization of this DPP extract using LC-HRMS revealed numerous flavonoids and phenols compounds which could be endowed with cardiopreventive actions. Overall, these results proved that DPP extract has preventive effects on cardiac remodeling process.

Original languageEnglish
Pages (from-to)656-665
Number of pages10
JournalExperimental and toxicologic pathology
Volume69
Issue number8
Early online date20 Jun 2017
DOIs
Publication statusE-pub ahead of print - 20 Jun 2017

Keywords

  • DPP ethanolic extract
  • Cardiopreventive
  • Myocardial infarction
  • ACE
  • ECG
  • Electrospray ionization mass spectrometry

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