Candelariella vitellina extract triggers in vitro and in vivo cell death through induction of apoptosis: a novel anticancer agent

Islam M. El-Garawani, Waill A. Elkhateeb, Gihan M. Zaghlol, Rafa S. Almeer, Eman F. Ahmed, Mostafa E. Rateb, Ahmed E. Abdel Moneim

Research output: Contribution to journalArticle

Abstract

Candelariella vitellina is common green-yellow lichen found on barks, wood, and rocks in Japanese forests. To investigate the mechanism of its anticancer potential, C. vitellina (80% MeOH/H2O) extract was prepared. High-performance liquid chromatography-high-resolution electrospray ionization mass spectrometry analysis revealed seven new compounds and 11 natural compounds of terpenes and polyketides. In vitro cytotoxicity analysis of Caco-2 cells exhibited an IC50 of 125 ± 4.1 μg/mL. No significant cytotoxicity was observed in vitro in normal human peripheral lymphocytes. Both the IC25 and IC50 were determined to explore the potent anticancer potential in this study. C. vitellina exhibited a mitochondrial P53-independent apoptotic effect with negative P53 expression and an elevated BAX/BCL2 ratio as well as upregulated CASP3 mRNA expression. Similarly, in vivo analysis showed the same pattern of anticancer potential but was dependent on the P53 expression. Furthermore, C. vitellina induced antioxidative conditions in vitro and in vivo. The decreased invasion of tumor cells in vivo and increased apoptotic features in vitro and in vivo suggest the moderate to strong apoptotic anticancer potential of C. vitellina. However, further studies are needed to determine the extent and mechanism of action on different cell lines to support the anticancer properties of this lichen.

LanguageEnglish
Pages110-119
Number of pages10
JournalFood and Chemical Toxicology
Volume127
Early online date7 Mar 2019
DOIs
StateE-pub ahead of print - 7 Mar 2019

Fingerprint

Antineoplastic Agents
Cell Death
Apoptosis
Lichens
Inhibitory Concentration 50
Polyketides
Caco-2 Cells
Electrospray Ionization Mass Spectrometry
Terpenes
Caspase 3
High Pressure Liquid Chromatography
Lymphocytes
Cell Line
Messenger RNA
In Vitro Techniques
Neoplasms

Keywords

  • Candelariella vitellina
  • Anticancer
  • Apoptosis
  • Terpenes
  • Polyketides

Cite this

El-Garawani, I. M., Elkhateeb, W. A., Zaghlol, G. M., Almeer, R. S., Ahmed, E. F., Rateb, M. E., & Abdel Moneim, A. E. (2019). Candelariella vitellina extract triggers in vitro and in vivo cell death through induction of apoptosis: a novel anticancer agent. Food and Chemical Toxicology, 127, 110-119. DOI: 10.1016/j.fct.2019.03.003
El-Garawani, Islam M. ; Elkhateeb, Waill A. ; Zaghlol, Gihan M. ; Almeer, Rafa S. ; Ahmed, Eman F. ; Rateb, Mostafa E. ; Abdel Moneim, Ahmed E./ Candelariella vitellina extract triggers in vitro and in vivo cell death through induction of apoptosis : a novel anticancer agent. In: Food and Chemical Toxicology. 2019 ; Vol. 127. pp. 110-119
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Candelariella vitellina extract triggers in vitro and in vivo cell death through induction of apoptosis : a novel anticancer agent. / El-Garawani, Islam M.; Elkhateeb, Waill A.; Zaghlol, Gihan M.; Almeer, Rafa S.; Ahmed, Eman F.; Rateb, Mostafa E.; Abdel Moneim, Ahmed E.

In: Food and Chemical Toxicology, Vol. 127, 31.05.2019, p. 110-119.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Candelariella vitellina extract triggers in vitro and in vivo cell death through induction of apoptosis

T2 - Food and Chemical Toxicology

AU - El-Garawani,Islam M.

AU - Elkhateeb,Waill A.

AU - Zaghlol,Gihan M.

AU - Almeer,Rafa S.

AU - Ahmed,Eman F.

AU - Rateb,Mostafa E.

AU - Abdel Moneim,Ahmed E.

N1 - Copyright © 2019 Elsevier Ltd. All rights reserved.

PY - 2019/3/7

Y1 - 2019/3/7

N2 - Candelariella vitellina is common green-yellow lichen found on barks, wood, and rocks in Japanese forests. To investigate the mechanism of its anticancer potential, C. vitellina (80% MeOH/H2O) extract was prepared. High-performance liquid chromatography-high-resolution electrospray ionization mass spectrometry analysis revealed seven new compounds and 11 natural compounds of terpenes and polyketides. In vitro cytotoxicity analysis of Caco-2 cells exhibited an IC50 of 125 ± 4.1 μg/mL. No significant cytotoxicity was observed in vitro in normal human peripheral lymphocytes. Both the IC25 and IC50 were determined to explore the potent anticancer potential in this study. C. vitellina exhibited a mitochondrial P53-independent apoptotic effect with negative P53 expression and an elevated BAX/BCL2 ratio as well as upregulated CASP3 mRNA expression. Similarly, in vivo analysis showed the same pattern of anticancer potential but was dependent on the P53 expression. Furthermore, C. vitellina induced antioxidative conditions in vitro and in vivo. The decreased invasion of tumor cells in vivo and increased apoptotic features in vitro and in vivo suggest the moderate to strong apoptotic anticancer potential of C. vitellina. However, further studies are needed to determine the extent and mechanism of action on different cell lines to support the anticancer properties of this lichen.

AB - Candelariella vitellina is common green-yellow lichen found on barks, wood, and rocks in Japanese forests. To investigate the mechanism of its anticancer potential, C. vitellina (80% MeOH/H2O) extract was prepared. High-performance liquid chromatography-high-resolution electrospray ionization mass spectrometry analysis revealed seven new compounds and 11 natural compounds of terpenes and polyketides. In vitro cytotoxicity analysis of Caco-2 cells exhibited an IC50 of 125 ± 4.1 μg/mL. No significant cytotoxicity was observed in vitro in normal human peripheral lymphocytes. Both the IC25 and IC50 were determined to explore the potent anticancer potential in this study. C. vitellina exhibited a mitochondrial P53-independent apoptotic effect with negative P53 expression and an elevated BAX/BCL2 ratio as well as upregulated CASP3 mRNA expression. Similarly, in vivo analysis showed the same pattern of anticancer potential but was dependent on the P53 expression. Furthermore, C. vitellina induced antioxidative conditions in vitro and in vivo. The decreased invasion of tumor cells in vivo and increased apoptotic features in vitro and in vivo suggest the moderate to strong apoptotic anticancer potential of C. vitellina. However, further studies are needed to determine the extent and mechanism of action on different cell lines to support the anticancer properties of this lichen.

KW - Candelariella vitellina

KW - Anticancer

KW - Apoptosis

KW - Terpenes

KW - Polyketides

U2 - 10.1016/j.fct.2019.03.003

DO - 10.1016/j.fct.2019.03.003

M3 - Article

VL - 127

SP - 110

EP - 119

JO - Food and Chemical Toxicology

JF - Food and Chemical Toxicology

SN - 0278-6915

ER -