Apicomplexan F‐actin is required for efficient nuclear entry during host cell invasion

Mario Del Rosario, Javier Periz, Georgios Pavlou, Oliver Lyth, Fernanda Latorre-Barragan, Sujaan Das, Gurman S. Pall, Johannes Felix Stortz, Leandro Lemgruber, Jamie A. Whitelaw, Jake Baum, Isabelle Tardieux, Markus Meissner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The obligate intracellular parasites Toxoplasma gondii and Plasmodium spp. invade host cells by injecting a protein complex into the membrane of the targeted cell that bridges the two cells through the assembly of a ring-like junction. This circular junction stretches while the parasites apply a traction force to pass through, a step that typically concurs with transient constriction of the parasite body. Here we analyse F-actin dynamics during host cell invasion. Super-resolution microscopy and real-time imaging highlighted an F-actin pool at the apex of pre-invading parasite, an F-actin ring at the junction area during invasion but also networks of perinuclear and posteriorly localised F-actin. Mutant parasites with dysfunctional acto-myosin showed significant decrease of junctional and perinuclear F-actin and are coincidently affected in nuclear passage through the junction. We propose that the F-actin machinery eases nuclear passage by stabilising the junction and pushing the nucleus through the constriction. Our analysis suggests that the junction opposes resistance to the passage of the parasite’s nucleus and provides the first evidence for a dual contribution of actin-forces during host cell invasion by apicomplexan parasites.
Original languageEnglish
Article numbere48896
Number of pages18
JournalEMBO reports
Volume2019
Issue number20
Early online date4 Oct 2019
DOIs
Publication statusPublished - 5 Dec 2019
Externally publishedYes

Keywords

  • actin
  • Apicomplexa
  • host cell invasion
  • myosin
  • nucleus

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