Abstract
Introduction
Dypsis decaryi seeds need more phytochemical and biological attention specially on one of the most important life-threatening problems (cancer).
Methods
Total ethanol extract of D. decaryi seeds (TEEDDS, Arecaceae) and its fixed oil fraction (FOF) were subjected to UPLC-HR-ESI-MS metabolic profiling and gas chromatography-mass spectrometry, respectively. Also, TEEDDS, FOF, and FOF silver nanoparticles (FOF-SNPs) were screened using 4 cell lines. FOF effects on expression of Bcl-2, Bax, P53, and epidermal growth factor receptor (EGFR) in human prostate carcinoma cell line (PC-3) were evaluated and supported by docking study.
Results
Metabolic profiling of TEEDDS resulted in identification of 18 compounds. While GC-MS of FOF displayed the presence of 12 fatty acids methyl esters. TEEDDS, staurosporine, FOF, and FOF-SNPs showed IC50= 3.55, 9.81, 16.88, and 0.01 µg/mL, respectively, against PC-3 (human prostate carcinoma cell line). FOF effects on the expression of Bcl-2, Bax, P53, and EGFR in PC-3 cell lines displayed IC50= 1.09, 220.6, 619.8, and 399.64 ng/mL, respectively. The FOF major constituent (E-13-octadecenoic acid) exhibited significant affinity and binding interactions that were comparable with that of the cocrystalized inhibitor.
Discussion/Conclusions
For the first time, in vitro antiproliferative activity against PC-3, HepG-2, HCT-116, and MCF-7 cells, and chemical profiles of TEEDDS and its FOF have been noteworthy studied. FOF and FOF-SNPs displayed the highest activities particularly on PC-3. Additionally, FOF showed significant activities on Bcl-2, Bax, P53, and EGFR, which was also supported by docking study. These results highlight that FOF and FOF-SNPs could have potent antiproliferative activity against PC-3 cell lines and may play an important role in antiproliferative drug development.
Dypsis decaryi seeds need more phytochemical and biological attention specially on one of the most important life-threatening problems (cancer).
Methods
Total ethanol extract of D. decaryi seeds (TEEDDS, Arecaceae) and its fixed oil fraction (FOF) were subjected to UPLC-HR-ESI-MS metabolic profiling and gas chromatography-mass spectrometry, respectively. Also, TEEDDS, FOF, and FOF silver nanoparticles (FOF-SNPs) were screened using 4 cell lines. FOF effects on expression of Bcl-2, Bax, P53, and epidermal growth factor receptor (EGFR) in human prostate carcinoma cell line (PC-3) were evaluated and supported by docking study.
Results
Metabolic profiling of TEEDDS resulted in identification of 18 compounds. While GC-MS of FOF displayed the presence of 12 fatty acids methyl esters. TEEDDS, staurosporine, FOF, and FOF-SNPs showed IC50= 3.55, 9.81, 16.88, and 0.01 µg/mL, respectively, against PC-3 (human prostate carcinoma cell line). FOF effects on the expression of Bcl-2, Bax, P53, and EGFR in PC-3 cell lines displayed IC50= 1.09, 220.6, 619.8, and 399.64 ng/mL, respectively. The FOF major constituent (E-13-octadecenoic acid) exhibited significant affinity and binding interactions that were comparable with that of the cocrystalized inhibitor.
Discussion/Conclusions
For the first time, in vitro antiproliferative activity against PC-3, HepG-2, HCT-116, and MCF-7 cells, and chemical profiles of TEEDDS and its FOF have been noteworthy studied. FOF and FOF-SNPs displayed the highest activities particularly on PC-3. Additionally, FOF showed significant activities on Bcl-2, Bax, P53, and EGFR, which was also supported by docking study. These results highlight that FOF and FOF-SNPs could have potent antiproliferative activity against PC-3 cell lines and may play an important role in antiproliferative drug development.
Original language | English |
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Article number | 100846 |
Journal | Journal of Herbal Medicine |
Volume | 44 |
Early online date | 26 Jan 2024 |
DOIs | |
Publication status | Published - 31 Mar 2024 |
Keywords
- Antiproliferative
- Arecaceae
- Dypsis decaryi (Neodypsis decaryi)
- Gas chromatography-mass spectrometry
- Metabolomic