Analysing the microbiome of diabetic foot ulcers

John Butcher, Eleanor Townsend, Karen Smith, Gordon Ramage, Craig Williams, Andrew Collier

Research output: Contribution to conferenceAbstractpeer-review

Abstract

Introduction: Diabetic foot ulcers (DFUs) are a major cause of
morbidity and mortality. The role of infection in the recurrence
and chronicity of DFUs is not well characterised, but is
recognised as an important determinant.
Objectives: This study aims to characterise the microbiome in
DFUs to determine whether or not key bacterial genera are
consistently represented or not.
Methods: DFUs of 16 patients with type II diabetes attending a
Diabetic Clinic at Ayr Hospital were swabbed. DNA from the
swabs was extracted using a combination of bead beating and
QIAmp mini DNA kit (Qiagen), then Next Generation Sequencing
(Illumina MiSeq) of the hypervariable V4 region of 16S rRNA was
performed.
Results: 216 unique operational taxonomic units (OTUs) were
identified in the sample set, with an mean of 42 per sample
(range 20 to 86). Of these, anaerobes (55%) dominated, followed
by facultative anaerobes (42%) and aerobes (3%), which came
from a variety of sources (environmental, skin commensals and
pathogens). The most common bacterium identified was the
anaerobe Finegoldia magna, with Streptococcus, Staphylococcus
and Anaerococcus species also isolated.
Conclusions: The microbiome of DFUs is heterogeneous and
dominated by anaerobic species, which has implication for
clinical management strategies.
Original languageEnglish
Pages138
Number of pages1
Publication statusPublished - 2015
EventSociety for General Microbiology Annual Conference 2015 - Birmingham, United Kingdom
Duration: 30 Mar 20152 Apr 2015

Conference

ConferenceSociety for General Microbiology Annual Conference 2015
CountryUnited Kingdom
CityBirmingham
Period30/03/152/04/15

Keywords

  • Diabetic foot ulcer
  • Microbiology
  • Biofilms

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