Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure: effect of haemodialysis and oxidative stress

Joanna Brzeszczynska, Marek Luciak, Krzysztof Gwozdzinski

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H2O2 among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H2O2 (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein–protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H2O2 exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.
Original languageEnglish
Pages (from-to)40-48
Number of pages9
JournalFree Radical Research
Volume42
Issue number1
DOIs
Publication statusPublished - 2008
Externally publishedYes

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Cellular Structures
Chronic Kidney Failure
Renal Dialysis
Oxidative Stress
Erythrocytes
Erythrocyte Membrane
Erythrocyte Aging
Osmotic Fragility
Membrane Fluidity
Dialysis
Spectrum Analysis

Keywords

  • Chronic renal failure
  • Hydrogen peroxide
  • Erythrocyte
  • Rheology
  • Cytoskeleton
  • Osmotic fragility

Cite this

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title = "Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure: effect of haemodialysis and oxidative stress",
abstract = "The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H2O2 among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H2O2 (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein–protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H2O2 exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.",
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Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure : effect of haemodialysis and oxidative stress. / Brzeszczynska, Joanna; Luciak, Marek; Gwozdzinski, Krzysztof.

In: Free Radical Research, Vol. 42, No. 1, 2008, p. 40-48.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure

T2 - effect of haemodialysis and oxidative stress

AU - Brzeszczynska, Joanna

AU - Luciak, Marek

AU - Gwozdzinski, Krzysztof

PY - 2008

Y1 - 2008

N2 - The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H2O2 among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H2O2 (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein–protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H2O2 exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.

AB - The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H2O2 among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H2O2 (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein–protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H2O2 exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.

KW - Chronic renal failure

KW - Hydrogen peroxide

KW - Erythrocyte

KW - Rheology

KW - Cytoskeleton

KW - Osmotic fragility

U2 - 10.1080/10715760701787693

DO - 10.1080/10715760701787693

M3 - Article

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JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 1

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