Alteration to the oral microbiome following dietary nitrate supplementation does not influence the vascular responsiveness to an acute nitrate dose

Mia Burleigh, Luke Liddle, Chris Monaghan, David J. Muggeridge, Nicholas Sculthorpe, John Butcher, Fiona Henriquez, Chris Easton

Research output: Contribution to journalMeeting Abstract

1 Citation (Scopus)

Abstract

Background 
Nitrate (NO3 -) contained in food and beverages can transiently increase nitric oxide (NO) availability following bacterial reduction to nitrite (NO2 -) in the oral cavity. Increased levels of NO have been consistently shown to reduce blood pressure and elicit other favourable cardiovascular and metabolic effects. We have previously reported that regular ingestion of dietary NO3 - increases salivary pH and alters the abundance of bacteria that are responsible for NO3 - reduction.

Purpose 
The present study tested the hypothesis that these adaptations to the oral bacteria would improve the capacity of the mouth to reduce NO3 - to NO2 - and increase the vascular responsiveness to a NO3 - dose.

Methods 
Eleven healthy males received 7 days of supplementation with NO3 --rich beetroot juice (BR). The acute responses following the ingestion of a bolus of BR were assessed before (day 0) and after (day 8) supplementation. Samples of saliva and blood were collected for the analysis of NO markers and vascular function was assessed by blood pressure and flow mediated dilation (FMD).

Results 
As previously reported, 7 days of supplementation with BR significantly altered the composition of the microbiome. Some species NO3 --reducing bacteria increased in abundance and other, mostly pathogenic species, were reduced. The ingestion of BR on days 0 and 8 increased salivary and plasma NO2 -, decreased blood pressure, and increased the FMD response (all p<0.05). Despite the alterations to the microbiome, there was no difference in the magnitude of the changes in: salivary NO2 - (day 0 Δ 2182.3 µM±1788.8 µM, day 8 Δ 1370.1 µM±1833.8 µM), plasma NO2 - (day 0 Δ 314.3 µM±149.7 µM, day 8 Δ 173.4±122.7 µM), flow mediated dilation (day 0 Δ 1.75%±1.71%, day 8 Δ 1.65%±2.12%), mean arterial blood pressure (day 0 Δ −1 mmHg ±4 mmHg, day 8 Δ −3 mmHg ±3 mmHg).

Conclusions 
Supplementing the diet with BR alters the microbial composition of the tongue in favour of oral health and results in transient improvements in vascular function. Nevertheless, the adaptations to the microbiome do not enhance the capacity to generate NO from dietary NO3 - or the vascular responsiveness to an acute bolus of BR.
Original languageEnglish
Article numberRW2
Pages (from-to)A4-A4
Number of pages1
JournalHeart
Volume105
Issue numberSuppl 4
Publication statusPublished - 1 May 2019

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Microbiota
Dietary Supplements
Nitrates
Blood Vessels
Nitric Oxide
Dilatation
Eating
Blood Pressure
Bacteria
Mouth
Arterial Pressure
Food and Beverages
Oral Health
Nitrites
Saliva
Tongue
Diet

Cite this

@article{96e068501629416a819235aa0e383dbb,
title = "Alteration to the oral microbiome following dietary nitrate supplementation does not influence the vascular responsiveness to an acute nitrate dose",
abstract = "Background Nitrate (NO3 -) contained in food and beverages can transiently increase nitric oxide (NO) availability following bacterial reduction to nitrite (NO2 -) in the oral cavity. Increased levels of NO have been consistently shown to reduce blood pressure and elicit other favourable cardiovascular and metabolic effects. We have previously reported that regular ingestion of dietary NO3 - increases salivary pH and alters the abundance of bacteria that are responsible for NO3 - reduction.Purpose The present study tested the hypothesis that these adaptations to the oral bacteria would improve the capacity of the mouth to reduce NO3 - to NO2 - and increase the vascular responsiveness to a NO3 - dose.Methods Eleven healthy males received 7 days of supplementation with NO3 --rich beetroot juice (BR). The acute responses following the ingestion of a bolus of BR were assessed before (day 0) and after (day 8) supplementation. Samples of saliva and blood were collected for the analysis of NO markers and vascular function was assessed by blood pressure and flow mediated dilation (FMD).Results As previously reported, 7 days of supplementation with BR significantly altered the composition of the microbiome. Some species NO3 --reducing bacteria increased in abundance and other, mostly pathogenic species, were reduced. The ingestion of BR on days 0 and 8 increased salivary and plasma NO2 -, decreased blood pressure, and increased the FMD response (all p<0.05). Despite the alterations to the microbiome, there was no difference in the magnitude of the changes in: salivary NO2 - (day 0 Δ 2182.3 µM±1788.8 µM, day 8 Δ 1370.1 µM±1833.8 µM), plasma NO2 - (day 0 Δ 314.3 µM±149.7 µM, day 8 Δ 173.4±122.7 µM), flow mediated dilation (day 0 Δ 1.75{\%}±1.71{\%}, day 8 Δ 1.65{\%}±2.12{\%}), mean arterial blood pressure (day 0 Δ −1 mmHg ±4 mmHg, day 8 Δ −3 mmHg ±3 mmHg).Conclusions Supplementing the diet with BR alters the microbial composition of the tongue in favour of oral health and results in transient improvements in vascular function. Nevertheless, the adaptations to the microbiome do not enhance the capacity to generate NO from dietary NO3 - or the vascular responsiveness to an acute bolus of BR.",
author = "Mia Burleigh and Luke Liddle and Chris Monaghan and Muggeridge, {David J.} and Nicholas Sculthorpe and John Butcher and Fiona Henriquez and Chris Easton",
year = "2019",
month = "5",
day = "1",
language = "English",
volume = "105",
pages = "A4--A4",
journal = "Heart",
issn = "1355-6037",
publisher = "BMJ Publishing Group",
number = "Suppl 4",

}

Alteration to the oral microbiome following dietary nitrate supplementation does not influence the vascular responsiveness to an acute nitrate dose. / Burleigh, Mia; Liddle, Luke; Monaghan, Chris; Muggeridge, David J.; Sculthorpe, Nicholas; Butcher, John; Henriquez, Fiona ; Easton, Chris.

In: Heart, Vol. 105, No. Suppl 4, RW2, 01.05.2019, p. A4-A4.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - Alteration to the oral microbiome following dietary nitrate supplementation does not influence the vascular responsiveness to an acute nitrate dose

AU - Burleigh, Mia

AU - Liddle, Luke

AU - Monaghan, Chris

AU - Muggeridge, David J.

AU - Sculthorpe, Nicholas

AU - Butcher, John

AU - Henriquez, Fiona

AU - Easton, Chris

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Background Nitrate (NO3 -) contained in food and beverages can transiently increase nitric oxide (NO) availability following bacterial reduction to nitrite (NO2 -) in the oral cavity. Increased levels of NO have been consistently shown to reduce blood pressure and elicit other favourable cardiovascular and metabolic effects. We have previously reported that regular ingestion of dietary NO3 - increases salivary pH and alters the abundance of bacteria that are responsible for NO3 - reduction.Purpose The present study tested the hypothesis that these adaptations to the oral bacteria would improve the capacity of the mouth to reduce NO3 - to NO2 - and increase the vascular responsiveness to a NO3 - dose.Methods Eleven healthy males received 7 days of supplementation with NO3 --rich beetroot juice (BR). The acute responses following the ingestion of a bolus of BR were assessed before (day 0) and after (day 8) supplementation. Samples of saliva and blood were collected for the analysis of NO markers and vascular function was assessed by blood pressure and flow mediated dilation (FMD).Results As previously reported, 7 days of supplementation with BR significantly altered the composition of the microbiome. Some species NO3 --reducing bacteria increased in abundance and other, mostly pathogenic species, were reduced. The ingestion of BR on days 0 and 8 increased salivary and plasma NO2 -, decreased blood pressure, and increased the FMD response (all p<0.05). Despite the alterations to the microbiome, there was no difference in the magnitude of the changes in: salivary NO2 - (day 0 Δ 2182.3 µM±1788.8 µM, day 8 Δ 1370.1 µM±1833.8 µM), plasma NO2 - (day 0 Δ 314.3 µM±149.7 µM, day 8 Δ 173.4±122.7 µM), flow mediated dilation (day 0 Δ 1.75%±1.71%, day 8 Δ 1.65%±2.12%), mean arterial blood pressure (day 0 Δ −1 mmHg ±4 mmHg, day 8 Δ −3 mmHg ±3 mmHg).Conclusions Supplementing the diet with BR alters the microbial composition of the tongue in favour of oral health and results in transient improvements in vascular function. Nevertheless, the adaptations to the microbiome do not enhance the capacity to generate NO from dietary NO3 - or the vascular responsiveness to an acute bolus of BR.

AB - Background Nitrate (NO3 -) contained in food and beverages can transiently increase nitric oxide (NO) availability following bacterial reduction to nitrite (NO2 -) in the oral cavity. Increased levels of NO have been consistently shown to reduce blood pressure and elicit other favourable cardiovascular and metabolic effects. We have previously reported that regular ingestion of dietary NO3 - increases salivary pH and alters the abundance of bacteria that are responsible for NO3 - reduction.Purpose The present study tested the hypothesis that these adaptations to the oral bacteria would improve the capacity of the mouth to reduce NO3 - to NO2 - and increase the vascular responsiveness to a NO3 - dose.Methods Eleven healthy males received 7 days of supplementation with NO3 --rich beetroot juice (BR). The acute responses following the ingestion of a bolus of BR were assessed before (day 0) and after (day 8) supplementation. Samples of saliva and blood were collected for the analysis of NO markers and vascular function was assessed by blood pressure and flow mediated dilation (FMD).Results As previously reported, 7 days of supplementation with BR significantly altered the composition of the microbiome. Some species NO3 --reducing bacteria increased in abundance and other, mostly pathogenic species, were reduced. The ingestion of BR on days 0 and 8 increased salivary and plasma NO2 -, decreased blood pressure, and increased the FMD response (all p<0.05). Despite the alterations to the microbiome, there was no difference in the magnitude of the changes in: salivary NO2 - (day 0 Δ 2182.3 µM±1788.8 µM, day 8 Δ 1370.1 µM±1833.8 µM), plasma NO2 - (day 0 Δ 314.3 µM±149.7 µM, day 8 Δ 173.4±122.7 µM), flow mediated dilation (day 0 Δ 1.75%±1.71%, day 8 Δ 1.65%±2.12%), mean arterial blood pressure (day 0 Δ −1 mmHg ±4 mmHg, day 8 Δ −3 mmHg ±3 mmHg).Conclusions Supplementing the diet with BR alters the microbial composition of the tongue in favour of oral health and results in transient improvements in vascular function. Nevertheless, the adaptations to the microbiome do not enhance the capacity to generate NO from dietary NO3 - or the vascular responsiveness to an acute bolus of BR.

M3 - Meeting Abstract

VL - 105

SP - A4-A4

JO - Heart

JF - Heart

SN - 1355-6037

IS - Suppl 4

M1 - RW2

ER -