Acute alcohol effects on cytokine concentrations in healthy young adults

A.J. Van De Loo, M.M. Mackus, A. Merlo, L. Habers, L.E. Devenney, K. Knipping, J. Garssen, A.D. Kraneveld, G. Bruce, J.C. Verster

Research output: Contribution to journalMeeting Abstract

Abstract

Background 
A common example of exposome pressure on immune fitness is the consumption of alcohol. In a previous study we demonstrated that (sic) the morning following an evening of heavy alcohol consumption (i.e. during alcohol hangover) significant increases of cytokine concentrations of IL-6 and IL-10 were observed. The aim of this study was to examine salivary cytokine concentrations over time in the first 7 hours directly after the consumption of alcohol.
Method N = 17 healthy young adults participated in an experimental study, comprising an alcohol test day and alcohol-free control day. A saliva sample was collected at baseline, i.e. before beverage consumption (alcohol or placebo) (timepoint 1). Subsequently, they consumed alcohol on the alcohol test day (to reach a BAC level of 0.08%) or received a placebo drink on the control day. Saliva was collected hourly for 7 hours, using the passive drool method (timepoint 2 to timepoint 8). Saliva concentrations of IL1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, GM-CSF, IFN-γ and TNF-α were assessed for each timepoint. Only those cytokines that could be reliably determined on multiple timepoints (<25% under the lower limit of detection) were used for statistical analysis. Outliers (>3SD difference from group average) were removed from the dataset. To correct for day-to-day variance in cytokine concentrations, baseline-corrected data were used for the analysis: the difference score (alcohol minus control) on T1 (baseline) was subtracted from all subsequent alcohol measurements (T2 to T8). GLM for repeated measures was used to compare cytokine concentrations on the alcohol and control day across the timepoints. Differences were considered significant when P < 0.05.
Results 
For N = 15 participants the salivary cytokine concentrations of IL-1β, IL-8, TNF - α could be reliably determined. Relative to the control day, significantly increased salivary cytokine concentrations were found on the alcohol day for IL-1β on time points T2 (P = 0.038), T3 (P = 0.048), and T8 (P = 0.0001). No significant differences were found for IL-8 and TNF-α.
Conclusion 
The current findings confirm that acute alcohol consumption has a direct increasing effect on cytokine concentrations in healthy young adults. However, the observed effects are of a much smaller magnitude as those seen when alcohol is consumed in the evening and cytokine concentrations are assessed the following morning, approximately 9 hours after stopping drinking.
Original languageEnglish
Pages (from-to)444-445
Number of pages2
JournalAllergy
Volume74
Issue numberS106
DOIs
Publication statusPublished - 8 Aug 2019

Cite this

Van De Loo, A. J., Mackus, M. M., Merlo, A., Habers, L., Devenney, L. E., Knipping, K., ... Verster, J. C. (2019). Acute alcohol effects on cytokine concentrations in healthy young adults. Allergy, 74(S106), 444-445. https://doi.org/10.1111/all.13961
Van De Loo, A.J. ; Mackus, M.M. ; Merlo, A. ; Habers, L. ; Devenney, L.E. ; Knipping, K. ; Garssen, J. ; Kraneveld, A.D. ; Bruce, G. ; Verster, J.C. / Acute alcohol effects on cytokine concentrations in healthy young adults. In: Allergy. 2019 ; Vol. 74, No. S106. pp. 444-445.
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title = "Acute alcohol effects on cytokine concentrations in healthy young adults",
abstract = "Background A common example of exposome pressure on immune fitness is the consumption of alcohol. In a previous study we demonstrated that (sic) the morning following an evening of heavy alcohol consumption (i.e. during alcohol hangover) significant increases of cytokine concentrations of IL-6 and IL-10 were observed. The aim of this study was to examine salivary cytokine concentrations over time in the first 7 hours directly after the consumption of alcohol.Method N = 17 healthy young adults participated in an experimental study, comprising an alcohol test day and alcohol-free control day. A saliva sample was collected at baseline, i.e. before beverage consumption (alcohol or placebo) (timepoint 1). Subsequently, they consumed alcohol on the alcohol test day (to reach a BAC level of 0.08{\%}) or received a placebo drink on the control day. Saliva was collected hourly for 7 hours, using the passive drool method (timepoint 2 to timepoint 8). Saliva concentrations of IL1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, GM-CSF, IFN-γ and TNF-α were assessed for each timepoint. Only those cytokines that could be reliably determined on multiple timepoints (<25{\%} under the lower limit of detection) were used for statistical analysis. Outliers (>3SD difference from group average) were removed from the dataset. To correct for day-to-day variance in cytokine concentrations, baseline-corrected data were used for the analysis: the difference score (alcohol minus control) on T1 (baseline) was subtracted from all subsequent alcohol measurements (T2 to T8). GLM for repeated measures was used to compare cytokine concentrations on the alcohol and control day across the timepoints. Differences were considered significant when P < 0.05.Results For N = 15 participants the salivary cytokine concentrations of IL-1β, IL-8, TNF - α could be reliably determined. Relative to the control day, significantly increased salivary cytokine concentrations were found on the alcohol day for IL-1β on time points T2 (P = 0.038), T3 (P = 0.048), and T8 (P = 0.0001). No significant differences were found for IL-8 and TNF-α.Conclusion The current findings confirm that acute alcohol consumption has a direct increasing effect on cytokine concentrations in healthy young adults. However, the observed effects are of a much smaller magnitude as those seen when alcohol is consumed in the evening and cytokine concentrations are assessed the following morning, approximately 9 hours after stopping drinking.",
author = "{Van De Loo}, A.J. and M.M. Mackus and A. Merlo and L. Habers and L.E. Devenney and K. Knipping and J. Garssen and A.D. Kraneveld and G. Bruce and J.C. Verster",
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Van De Loo, AJ, Mackus, MM, Merlo, A, Habers, L, Devenney, LE, Knipping, K, Garssen, J, Kraneveld, AD, Bruce, G & Verster, JC 2019, 'Acute alcohol effects on cytokine concentrations in healthy young adults', Allergy, vol. 74, no. S106, pp. 444-445. https://doi.org/10.1111/all.13961

Acute alcohol effects on cytokine concentrations in healthy young adults. / Van De Loo, A.J.; Mackus, M.M.; Merlo, A.; Habers, L.; Devenney, L.E.; Knipping, K.; Garssen, J.; Kraneveld, A.D.; Bruce, G.; Verster, J.C.

In: Allergy, Vol. 74, No. S106, 08.08.2019, p. 444-445.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - Acute alcohol effects on cytokine concentrations in healthy young adults

AU - Van De Loo, A.J.

AU - Mackus, M.M.

AU - Merlo, A.

AU - Habers, L.

AU - Devenney, L.E.

AU - Knipping, K.

AU - Garssen, J.

AU - Kraneveld, A.D.

AU - Bruce, G.

AU - Verster, J.C.

PY - 2019/8/8

Y1 - 2019/8/8

N2 - Background A common example of exposome pressure on immune fitness is the consumption of alcohol. In a previous study we demonstrated that (sic) the morning following an evening of heavy alcohol consumption (i.e. during alcohol hangover) significant increases of cytokine concentrations of IL-6 and IL-10 were observed. The aim of this study was to examine salivary cytokine concentrations over time in the first 7 hours directly after the consumption of alcohol.Method N = 17 healthy young adults participated in an experimental study, comprising an alcohol test day and alcohol-free control day. A saliva sample was collected at baseline, i.e. before beverage consumption (alcohol or placebo) (timepoint 1). Subsequently, they consumed alcohol on the alcohol test day (to reach a BAC level of 0.08%) or received a placebo drink on the control day. Saliva was collected hourly for 7 hours, using the passive drool method (timepoint 2 to timepoint 8). Saliva concentrations of IL1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, GM-CSF, IFN-γ and TNF-α were assessed for each timepoint. Only those cytokines that could be reliably determined on multiple timepoints (<25% under the lower limit of detection) were used for statistical analysis. Outliers (>3SD difference from group average) were removed from the dataset. To correct for day-to-day variance in cytokine concentrations, baseline-corrected data were used for the analysis: the difference score (alcohol minus control) on T1 (baseline) was subtracted from all subsequent alcohol measurements (T2 to T8). GLM for repeated measures was used to compare cytokine concentrations on the alcohol and control day across the timepoints. Differences were considered significant when P < 0.05.Results For N = 15 participants the salivary cytokine concentrations of IL-1β, IL-8, TNF - α could be reliably determined. Relative to the control day, significantly increased salivary cytokine concentrations were found on the alcohol day for IL-1β on time points T2 (P = 0.038), T3 (P = 0.048), and T8 (P = 0.0001). No significant differences were found for IL-8 and TNF-α.Conclusion The current findings confirm that acute alcohol consumption has a direct increasing effect on cytokine concentrations in healthy young adults. However, the observed effects are of a much smaller magnitude as those seen when alcohol is consumed in the evening and cytokine concentrations are assessed the following morning, approximately 9 hours after stopping drinking.

AB - Background A common example of exposome pressure on immune fitness is the consumption of alcohol. In a previous study we demonstrated that (sic) the morning following an evening of heavy alcohol consumption (i.e. during alcohol hangover) significant increases of cytokine concentrations of IL-6 and IL-10 were observed. The aim of this study was to examine salivary cytokine concentrations over time in the first 7 hours directly after the consumption of alcohol.Method N = 17 healthy young adults participated in an experimental study, comprising an alcohol test day and alcohol-free control day. A saliva sample was collected at baseline, i.e. before beverage consumption (alcohol or placebo) (timepoint 1). Subsequently, they consumed alcohol on the alcohol test day (to reach a BAC level of 0.08%) or received a placebo drink on the control day. Saliva was collected hourly for 7 hours, using the passive drool method (timepoint 2 to timepoint 8). Saliva concentrations of IL1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, GM-CSF, IFN-γ and TNF-α were assessed for each timepoint. Only those cytokines that could be reliably determined on multiple timepoints (<25% under the lower limit of detection) were used for statistical analysis. Outliers (>3SD difference from group average) were removed from the dataset. To correct for day-to-day variance in cytokine concentrations, baseline-corrected data were used for the analysis: the difference score (alcohol minus control) on T1 (baseline) was subtracted from all subsequent alcohol measurements (T2 to T8). GLM for repeated measures was used to compare cytokine concentrations on the alcohol and control day across the timepoints. Differences were considered significant when P < 0.05.Results For N = 15 participants the salivary cytokine concentrations of IL-1β, IL-8, TNF - α could be reliably determined. Relative to the control day, significantly increased salivary cytokine concentrations were found on the alcohol day for IL-1β on time points T2 (P = 0.038), T3 (P = 0.048), and T8 (P = 0.0001). No significant differences were found for IL-8 and TNF-α.Conclusion The current findings confirm that acute alcohol consumption has a direct increasing effect on cytokine concentrations in healthy young adults. However, the observed effects are of a much smaller magnitude as those seen when alcohol is consumed in the evening and cytokine concentrations are assessed the following morning, approximately 9 hours after stopping drinking.

U2 - 10.1111/all.13961

DO - 10.1111/all.13961

M3 - Meeting Abstract

VL - 74

SP - 444

EP - 445

JO - Allergy

JF - Allergy

SN - 0105-4538

IS - S106

ER -

Van De Loo AJ, Mackus MM, Merlo A, Habers L, Devenney LE, Knipping K et al. Acute alcohol effects on cytokine concentrations in healthy young adults. Allergy. 2019 Aug 8;74(S106):444-445. https://doi.org/10.1111/all.13961