A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria

Research output: Contribution to conferenceAbstract

Abstract

Background
Chronic wounds, such as diabetic foot ulcers, are commonly infected leading to recurrence and chronicity of the wounds, although this is not well characterised. We have previously developed a simple novel three-dimensional in vitro interkingdom wound biofilm model that has been used to characterise the response to wound washes. Here, the complexity of the model was increased to include anaerobes species found within our recent diabetic wound microbiome analysis.
MethodsThese biofilm consortia consisted of the yeast, Candida albicans, the aerobes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus hominis, Streptococcus agalactiae, and Corynebacterium simulans. The anaerobes included were Finegoldia magna, Anaerococcus vaginalis, Peptoniphilus gorbachii, Porphyromnas asacharolytica, and Prevotella buccalis. Biofilms were grown over a period of 9 days within a cellulose matrix hydrogel, with intermittent sampling. Compositional qPCR was used to enumerate each species within the biofilm. 
ResultsOverall, approximately 108 bacteria colonised the cellulose matrix, comprised of yeasts, aerobes and anaerobes. Aerobic bacteria were reduced by approximately 2 logs when the biofilm was grown in anaerobic conditions compared to 5% CO2 or aerobically (p<0.01). Bacterial species were shown to coaggregate with the yeast and hyphae, supporting their adhesion and biofilm formation.
ConclusionThe addition of anaerobes to the wound model further reflects the environment in vivo, allowing this model to be used for accurate testing of antimicrobial agents. The ability of C. albicans to support interkingdom interactions, as well as anaerobes in a non-anoxic environment may explain why we observe elevated levels of anaerobes in these diabetic wounds.
Original languageEnglish
Publication statusPublished - 3 Apr 2017
EventMicrobiology Society Annual Conference 2017 - Edinburgh International Conference Centre, Edinburgh, United Kingdom
Duration: 3 Apr 20176 Apr 2017
https://www.microbiologysociety.org/event/annual-conference-2017.html

Conference

ConferenceMicrobiology Society Annual Conference 2017
Abbreviated titleMicrobio17
CountryUnited Kingdom
CityEdinburgh
Period3/04/176/04/17
Internet address

Fingerprint

Anaerobic Bacteria
Biofilms
Wounds and Injuries
Growth
Yeasts
Candida albicans
Cellulose
Staphylococcus hominis
Prevotella
Corynebacterium
Aerobic Bacteria
Diabetic Foot
Streptococcus agalactiae
Hyphae
Hydrogel
Microbiota
Anti-Infective Agents
In Vitro Techniques
Pseudomonas aeruginosa
Staphylococcus aureus

Keywords

  • Microbiology
  • Diabetic Foot Wound
  • biofilm infections

Cite this

Townsend, E., MacKay, W., Williams, C., & Ramage, G. (2017). A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria. Abstract from Microbiology Society Annual Conference 2017, Edinburgh, United Kingdom.
Townsend, Eleanor ; MacKay, William ; Williams, Craig ; Ramage, Gordon. / A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria. Abstract from Microbiology Society Annual Conference 2017, Edinburgh, United Kingdom.
@conference{a0898098e0844308b3c661adb85046c8,
title = "A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria",
abstract = "BackgroundChronic wounds, such as diabetic foot ulcers, are commonly infected leading to recurrence and chronicity of the wounds, although this is not well characterised. We have previously developed a simple novel three-dimensional in vitro interkingdom wound biofilm model that has been used to characterise the response to wound washes. Here, the complexity of the model was increased to include anaerobes species found within our recent diabetic wound microbiome analysis.MethodsThese biofilm consortia consisted of the yeast, Candida albicans, the aerobes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus hominis, Streptococcus agalactiae, and Corynebacterium simulans. The anaerobes included were Finegoldia magna, Anaerococcus vaginalis, Peptoniphilus gorbachii, Porphyromnas asacharolytica, and Prevotella buccalis. Biofilms were grown over a period of 9 days within a cellulose matrix hydrogel, with intermittent sampling. Compositional qPCR was used to enumerate each species within the biofilm. ResultsOverall, approximately 108 bacteria colonised the cellulose matrix, comprised of yeasts, aerobes and anaerobes. Aerobic bacteria were reduced by approximately 2 logs when the biofilm was grown in anaerobic conditions compared to 5{\%} CO2 or aerobically (p<0.01). Bacterial species were shown to coaggregate with the yeast and hyphae, supporting their adhesion and biofilm formation.ConclusionThe addition of anaerobes to the wound model further reflects the environment in vivo, allowing this model to be used for accurate testing of antimicrobial agents. The ability of C. albicans to support interkingdom interactions, as well as anaerobes in a non-anoxic environment may explain why we observe elevated levels of anaerobes in these diabetic wounds.",
keywords = "Microbiology, Diabetic Foot Wound, biofilm infections",
author = "Eleanor Townsend and William MacKay and Craig Williams and Gordon Ramage",
year = "2017",
month = "4",
day = "3",
language = "English",
note = "Microbiology Society Annual Conference 2017, Microbio17 ; Conference date: 03-04-2017 Through 06-04-2017",
url = "https://www.microbiologysociety.org/event/annual-conference-2017.html",

}

Townsend, E, MacKay, W, Williams, C & Ramage, G 2017, 'A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria' Microbiology Society Annual Conference 2017, Edinburgh, United Kingdom, 3/04/17 - 6/04/17, .

A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria. / Townsend, Eleanor; MacKay, William; Williams, Craig; Ramage, Gordon.

2017. Abstract from Microbiology Society Annual Conference 2017, Edinburgh, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria

AU - Townsend, Eleanor

AU - MacKay, William

AU - Williams, Craig

AU - Ramage, Gordon

PY - 2017/4/3

Y1 - 2017/4/3

N2 - BackgroundChronic wounds, such as diabetic foot ulcers, are commonly infected leading to recurrence and chronicity of the wounds, although this is not well characterised. We have previously developed a simple novel three-dimensional in vitro interkingdom wound biofilm model that has been used to characterise the response to wound washes. Here, the complexity of the model was increased to include anaerobes species found within our recent diabetic wound microbiome analysis.MethodsThese biofilm consortia consisted of the yeast, Candida albicans, the aerobes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus hominis, Streptococcus agalactiae, and Corynebacterium simulans. The anaerobes included were Finegoldia magna, Anaerococcus vaginalis, Peptoniphilus gorbachii, Porphyromnas asacharolytica, and Prevotella buccalis. Biofilms were grown over a period of 9 days within a cellulose matrix hydrogel, with intermittent sampling. Compositional qPCR was used to enumerate each species within the biofilm. ResultsOverall, approximately 108 bacteria colonised the cellulose matrix, comprised of yeasts, aerobes and anaerobes. Aerobic bacteria were reduced by approximately 2 logs when the biofilm was grown in anaerobic conditions compared to 5% CO2 or aerobically (p<0.01). Bacterial species were shown to coaggregate with the yeast and hyphae, supporting their adhesion and biofilm formation.ConclusionThe addition of anaerobes to the wound model further reflects the environment in vivo, allowing this model to be used for accurate testing of antimicrobial agents. The ability of C. albicans to support interkingdom interactions, as well as anaerobes in a non-anoxic environment may explain why we observe elevated levels of anaerobes in these diabetic wounds.

AB - BackgroundChronic wounds, such as diabetic foot ulcers, are commonly infected leading to recurrence and chronicity of the wounds, although this is not well characterised. We have previously developed a simple novel three-dimensional in vitro interkingdom wound biofilm model that has been used to characterise the response to wound washes. Here, the complexity of the model was increased to include anaerobes species found within our recent diabetic wound microbiome analysis.MethodsThese biofilm consortia consisted of the yeast, Candida albicans, the aerobes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus hominis, Streptococcus agalactiae, and Corynebacterium simulans. The anaerobes included were Finegoldia magna, Anaerococcus vaginalis, Peptoniphilus gorbachii, Porphyromnas asacharolytica, and Prevotella buccalis. Biofilms were grown over a period of 9 days within a cellulose matrix hydrogel, with intermittent sampling. Compositional qPCR was used to enumerate each species within the biofilm. ResultsOverall, approximately 108 bacteria colonised the cellulose matrix, comprised of yeasts, aerobes and anaerobes. Aerobic bacteria were reduced by approximately 2 logs when the biofilm was grown in anaerobic conditions compared to 5% CO2 or aerobically (p<0.01). Bacterial species were shown to coaggregate with the yeast and hyphae, supporting their adhesion and biofilm formation.ConclusionThe addition of anaerobes to the wound model further reflects the environment in vivo, allowing this model to be used for accurate testing of antimicrobial agents. The ability of C. albicans to support interkingdom interactions, as well as anaerobes in a non-anoxic environment may explain why we observe elevated levels of anaerobes in these diabetic wounds.

KW - Microbiology

KW - Diabetic Foot Wound

KW - biofilm infections

M3 - Abstract

ER -

Townsend E, MacKay W, Williams C, Ramage G. A novel in vitro interkingdom wound biofilm model can support the growth of anaerobic bacteria. 2017. Abstract from Microbiology Society Annual Conference 2017, Edinburgh, United Kingdom.