TY - JOUR
T1 - A method for determination of hematocrit using the mobile app “HaemoCalc”
T2 - validity, reliability, and effect of user expertise
AU - Hayes, Lawrence D.
AU - Sanal-Hayes, Nilihan E. M.
AU - Ellam, Maryam
AU - Mclaughlin, Marie
AU - Swainson, Michelle G.
AU - Sculthorpe, Nicholas F.
PY - 2025/4/15
Y1 - 2025/4/15
N2 - We evaluated validity, reliability, and effect of user expertise of “HaemoCalc”, a mobile phone application for hematocrit (Hct) measurement from fingerpick blood samples, compared to a traditional Hawksley microhaematocrit reader (MHR). Experiment 1 examined the effect pitch angle during image capture exerted on the validity of Hct values. Twenty participants' samples were analyzed at 0°, 10°, and 20° directly over the sample, and 33° with a 10 cm setback. Analysis of variance (ANOVA) revealed a significant effect of angle on Hct values (p < 0.01). Measurements at 33° pitch differed from other angles and the MHR (p < 0.001, d = 2.31–3.06). Bland–Altman analysis showed good agreement at 0°, 10°, and 20° (mean differences: −0.4% to 1.0%) but poor agreement at 33° (mean difference: −4.4%, LOA: −0.7% to 8.4%). Experiment 2 assessed inter- and intra-rater reliability of expert and novice users (n = 12). Participants performed three trials each. HaemoCalc and MHR showed excellent reliability (ICC = 0.95–1.00). No differences were observed between experts and novices (p = 1.000, d = 0.01–0.39). HaemoCalc is a valid and reliable tool for Hct measurement at small pitch angles and in expert and novice users. The HaemoCalc app offers scalability, repeatability, health and safety benefits, and potential applications in medical education and remote learning.
AB - We evaluated validity, reliability, and effect of user expertise of “HaemoCalc”, a mobile phone application for hematocrit (Hct) measurement from fingerpick blood samples, compared to a traditional Hawksley microhaematocrit reader (MHR). Experiment 1 examined the effect pitch angle during image capture exerted on the validity of Hct values. Twenty participants' samples were analyzed at 0°, 10°, and 20° directly over the sample, and 33° with a 10 cm setback. Analysis of variance (ANOVA) revealed a significant effect of angle on Hct values (p < 0.01). Measurements at 33° pitch differed from other angles and the MHR (p < 0.001, d = 2.31–3.06). Bland–Altman analysis showed good agreement at 0°, 10°, and 20° (mean differences: −0.4% to 1.0%) but poor agreement at 33° (mean difference: −4.4%, LOA: −0.7% to 8.4%). Experiment 2 assessed inter- and intra-rater reliability of expert and novice users (n = 12). Participants performed three trials each. HaemoCalc and MHR showed excellent reliability (ICC = 0.95–1.00). No differences were observed between experts and novices (p = 1.000, d = 0.01–0.39). HaemoCalc is a valid and reliable tool for Hct measurement at small pitch angles and in expert and novice users. The HaemoCalc app offers scalability, repeatability, health and safety benefits, and potential applications in medical education and remote learning.
KW - hematocrit
KW - limits of detection
KW - medical education
KW - mobile app
KW - red blood cells
KW - software validation
U2 - 10.14814/phy2.70314
DO - 10.14814/phy2.70314
M3 - Article
SN - 2051-817X
VL - 13
JO - Physiological Reports
JF - Physiological Reports
IS - 8
M1 - e70314
ER -