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Personal profile

Area of academic expertise - outline

Cardiovascular disease is the single leading cause of death and disability in the world (responsible for almost 1 in every 3 deaths). Dysfunction of endothelial cells, a cell type that lines the surface of every blood vessel, plays a critical role in the development and progression of cardiovascular disease but as yet we do not have effective therapeutic means to protect or restore activity of this key cell type. My research focuses on the role of endothelial cells as a regulator of vascular smooth muscle cell activity and how this can be is disrupted by the inflammatory conditions that occur in diseases such as obesity, diabetes mellitus, preeclampsia and rheumatoid arthritis. Understanding more about how endothelial cells become dysfunctional will greatly enhance our insight into the cellular processes that underlie the cardiovascular disease and help find means to prevent the its devastating consequences, such as heart attack and stroke.

I have established an ex vivo vascular assessment suite allowing the determination of both macro- and micro-vascular reactivity i.e. real-time recording of contraction and relaxation of isolated blood vessels. The key advantage of ex vivo analysis of vascular reactivity is that the blood vessel is free from hormonal or neural influence and in an environment which allows for accurate probing of cellular pathways. Furthermore, through my partnership with the Centre of Musculoskeletal Science at Glasgow University, we can also determine blood flow in vivo with use of a laser Doppler technique. Moreover, access to techniques such as PCR, immunohistochemistry, western blotting and ELISA allows for a multi-faceted multi-disciplinary approach to exploring vascular dysfunction.

Current research activities

My current research investigates the mechanisms underlying vascular dysfunction found in several diverse disorders. Specifically we are exploring the role of the angiotensin 2 type 1 receptor as a mediator of vascular dysfunction found in rheumatoid arthritis. Furthermore we are analysing the role of adipose tissue as a regulator of vascular reactivity and how with can be modified by proteinase-activated receptor 2 in conditions of obesity. In addition we are analysing human placental artery function in relation to interleukin levels to investigate potential a route by which preeclampsia may develop. Moreover we are examining flavonoids and other dietary supplements for their putative protective action against the detrimental influence of glucose vascular reactivity.

Desired research direction

My aim is to garner further insight into the cellular mechanisms underlying vascular disorder and to utilise this knowledge to highlight novel routes of therapy and, along with industrial partners, develop targeted pharmaceutical interventions to prevent or alleviate this most devastating of diseases.

Keywords

  • QP Physiology
  • Cardiovascular disease
  • Endothelium
  • Nitric Oxide
  • AT1R
  • Inflammation

Fingerprint Fingerprint is based on mining the text of the person's scientific documents to create an index of weighted terms, which defines the key subjects of each individual researcher.

  • 9 Similar Profiles
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Endothelium Medicine & Life Sciences
Blood Vessels Medicine & Life Sciences
Experimental Arthritis Medicine & Life Sciences
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Aorta Medicine & Life Sciences
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Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 2008 2018

  • 5 Article
  • 2 Conference contribution
  • 2 Poster
  • 1 Meeting Abstract

Assessment of the effects of exercise on a murine model of OA

Huesa, C., Dunning, L., Macdougall, K., Ortiz, A., Villaluz, M. M., Fegen, M., Crilly, A., Litherland, G., MacKenzie, A., van't Hof, R., Goodyear, C. & Lockhart, J., 8 Jan 2018, (Accepted/In press).

Research output: Contribution to conferencePoster

Tibial Meniscus
Osteoarthritis
Exercise
Joints
Osteosclerosis
3 Downloads (Pure)
Open Access
File
Angiotensin Type 1 Receptor
Freund's Adjuvant
Endothelium
Rheumatoid Arthritis
Losartan

Gender specific generation of nitroxyl (HNO) from rat endothelium

Hamilton, K. & MacKenzie, A., Aug 2015, In : Vascular Pharmacology. 71, p. 208-214

Research output: Contribution to journalArticle

Sex differences in vascular endothelial cell function reveal a gender specific role for nitroxyl

Hamilton, K. & MacKenzie, A., 2012.

Research output: Contribution to conferencePoster

Open Access
Sex Characteristics
Endothelial Cells
Phenylephrine
Blood Vessels
Aorta

Prizes

pharmacology
certification

Activities 2012 2019

Meeting with Glasgow Respiratory Clinicians

John Lockhart (Participant), Andrew MacKenzie (Organiser), Gary Litherland (Participant), Anne Crilly (Participant), William MacKay (Participant), Craig Williams (Participant), Joanna Brzeszczynska (Participant), Mariarca Bailo (Presenter), Kirsty McCallum (Presenter), Bryn Short (Presenter), Kimberly Black (Presenter), Fawziye Tarhini (Presenter), David Anderson (Participant), Hannah Bayes (Participant), Pallavai Bedi (Participant)
8 Feb 2019

Activity: Other

PhD External Examiner University of Kingston

Andrew MacKenzie (Examiner)
31 May 2018

Activity: Examination

School visit - Bargeddie Primary School

Andrew MacKenzie (Speaker)
Mar 2018

Activity: OtherTypes of Public engagement and outreach - Schools engagement

BREATH Annual Conference

John Lockhart (Invited speaker), Gary Litherland (Invited speaker), Anne Crilly (Invited speaker), Andrew MacKenzie (Invited speaker), Iain McLellan (Invited speaker), Andisheh Bakhshi (Invited speaker), Mark Thomas (Invited speaker), Mariarca Bailo (Invited speaker), Kimberly Black (Invited speaker), Kirsty McCallum (Invited speaker)
19 Jun 201820 Jun 2018

Activity: Participating in or organising an eventParticipation in conference

Profiles

PAR-2 Receptor
Premature Aging
Autophagy
Lung
Chronic Obstructive Pulmonary Disease
Tibial Meniscus
Osteoarthritis
Exercise
Joints
Osteosclerosis
Tibial Meniscus
Osteoarthritis
Exercise
Joints
Osteosclerosis